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Figure S1. Characterisation of ZnO-NPs by (a) XRD plot and (b) TEM imaging showing NPs with mean diameter of 30 nm.

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Figure S2. Antifungal activities of (1) ZnO-NPs, (2) ZnO, (3) ZnSO4 and (4) Zn(ClO4)2 against (a) F. graminearum (b) A. flavus and (c) P. citrinum on PDA at different concentrations (data are shown as average values and standard deviation of diameter fungal). All treatments were statistically significant when compared with Control group (P < 0.05).

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Figure S3. Antimycotoxin activities of Zn-compounds treated (100 mM) on toxigenic F. graminearum (DON), A. flavus (AFB1), P. citrinum (CTR) and (a) showing TLC plates with mycotoxins by metodology; (b) data are shown as average values and standard deviation of mycotoxin intensity (arbitrary units – a.u.). Symbols indicate statistically significant when compared with Control group *P < 0.05.

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Figure S4. Conidia production of F. graminearum, A. flavus and P. citrinum on Zn-compounds media treated (100 mM). Data are shown as average values and standard deviation of log (conidia number per mL). Symbols indicate statistically significant when compared to Control group *P < 0.001.

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Figure S5. Effect of the Zn-compounds treatments on (a) F. graminearum, (b) A. flavus and (c) P. citrinum hyphae. [Control: no treatment; zinc- compounds treated (100 mM): (1) ZnO-NPs, (2) ZnO, (3) ZnSO4, (4) Zn(ClO4)2, showing hyphae alterations]. SEM micrographs images in size of 93000.

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Figure S6. Effect of the Evans Blue dye distribution in the (a) F.graminearum, (b) A. flavus and (c) P. citrinum hyphae. [Control: no treatment – without coloration (hyphae alive); Zn-compounds treated (100 mM): (1) ZnO-NPs, (2) ZnO, (3) ZnSO4, (4) Zn (ClO4)2, showing hyphae coloration (hyphae dead). OM images at 9400.

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Figure S7. Effect of the H2DCFDA on (a) F. graminearum, (b) A. flavus and (c) P. citrinum hyphae. [Control: no treatment (no ROS production); Zn-compounds treated (100 mM): (1) ZnO-NPs, (2) ZnO, (3) ZnSO4, (4) Zn(ClO4)2, showing hyphae fluorescence, development after ROS production]. COM images at 9300.

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