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ijfs12475-sup-0001-FigS1.docWord document74KFigure S1. Change in the (a) total polyphenol content; (b) total flavonoid content; (c) reducing sugar content; (d) iron chelating activity of Oenothera biennis, Borago officinalis, Nigella sativa seedcake extracts before and after enzymatic hydrolysis.
ijfs12475-sup-0002-FigS2.docxWord document60KFigure S2. Change of (a) superoxide anion radical-scavenging capacity; (b) ABTS radical-scavenging capacity; (c) DPPH radical-scavenging capacity of Oenothera biennis, Borago officinalis, Nigella sativa seedcake extracts before and after enzymatic hydrolysis and reference substance catechin.
ijfs12475-sup-0003-FigS3.docxWord document18KFigure S3. Correlation between the increase in the (a) polyphenol content and DPPH antioxidant activity; (b) polyphenol content and ABTS antioxidant activity of ethanolic extract from Oenothera biennis, Borago officinalis, Nigella sativa seedcake before and after enzymatic hydrolysis. Pearson's correlation coefficients (r2).
ijfs12475-sup-0004-FigS4.docWord document87KFigure S4. Change of A-elastase, B-collagenase, C-lipoxygenase inhibition activity of Oenothera biennis, Borago officinalis, Nigella sativa seedcake extracts before and after enzymatic hydrolysis and reference substance catechin.
ijfs12475-sup-0005-FigS5.docxWord document99KFigure S5. HPLC-DAD chromatograms of phenolic compounds in: (a) Oenothera biennis seedcake extract before enzymatic hydrolysis 1 – gallic acid, 2-quercetin, 3-catechin, 4-ferulic acid; (b) Borago officinalis seedcake extract before enzymatic hydrolysis 1- gallic acid, 2-quercetin, 3-catechin; (c) Nigella sativa seedcake extract before enzymatic hydrolysis 1-quercetin, 2-hydroxybenzoic acid, 3-syringic acid; (d) Oenothera biennis seedcake extract after enzymatic hydrolysis 1 – gallic acid, 2-quercetin, 3-catechin, 4-ferulic acid; (e) Borago officinalis seedcake extract after enzymatic hydrolysis 1- gallic acid, 2-quercetin, 3-catechin; (f) Nigella sativa seedcake extract after enzymatic hydrolysis 1-quercetin, 2-hydroxybenzoic acid, 3-syringic acid.
ijfs12475-sup-0006-TableS1-S3.docxWord document17K

Table S1. The compounds from the Oenothera biennis, Borago officinalis and Nigella sativa seedcake extracts.

Table S2. Antimicrobial activity of Oenothera biennis, Borago officinalis and Nigella sativa seedcake ethanol extract before and after enzymatic hydrolysis (10 μL) in agar disc diffusion method.

Table S3. HPLC-DAD analysis. Retention time tR [min]and UV spectral data λmax [nm] for standard compounds.

ijfs12475-sup-0007-legends.docxWord document13K 

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