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Molecular cloning, sequencing and structural studies of granulocyte–macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis)

Authors

  • Thennarasu Sugumar,

    Corresponding author
    1. Bioscience Core Lab, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia
    • Correspondence: Thennarasu Sugumar, Bioscience Core Lab, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia. Tel: +966 28082463; Fax: +966 28020059; E-mail: thenna1979@hotmail.com

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  • Ganesan Pugalenthi,

    1. Bioscience Core Lab, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia
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  • Murugesan Harishankar,

    1. Department of Immunology, Tuberculosis Research Centre, Indian Council of Medical Research, Chennai, India
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  • G Dhinakar Raj

    1. Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India
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Summary

Granulocyte–macrophage colony-stimulating factor (GM-CSF) is a cytokine that is essential for growth and development of progenitors of granulocytes and monocytes/macrophages. In this study, we report molecular cloning, sequencing and characterization of GM-CSF from Indian water buffalo, Bubalus bubalis. In addition, we performed sequence and structural analysis for buffalo GM-CSF. Buffalo GM-CSF has been compared with 17 mammalian GM-CSFs using multiple sequence alignment and phylogenetic tree. Three-dimensional model for buffalo GM-CSF and human receptor complex was built using homology modelling to study cross-reactivity between two species. Detailed analysis was performed to study GM-CSF interface and various interactions at the interface.

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