Z. B. R. and T. R. contributed equally to this paper.
Differential effects of paracrine factors on the survival of cells of the neurovascular unit during oxygen glucose deprivation
Article first published online: 10 NOV 2013
© 2013 The Authors. International Journal of Stroke © 2013 World Stroke Organization
International Journal of Stroke
Volume 10, Issue 3, pages 407–414, April 2015
How to Cite
Redzic, Z. B., Rabie, T., Sutherland, B. A. and Buchan, A. M. (2015), Differential effects of paracrine factors on the survival of cells of the neurovascular unit during oxygen glucose deprivation. International Journal of Stroke, 10: 407–414. doi: 10.1111/ijs.12197
Conflicts of interest: None declared.
- Issue published online: 16 MAR 2015
- Article first published online: 10 NOV 2013
- Manuscript Accepted: 5 AUG 2013
- Manuscript Received: 15 MAY 2013
- Henry Smith Charity
- Fondation Leducq
- brain endothelial cells;
- neurovascular unit;
- oxygen glucose deprivation;
- transforming growth factor β1;
- vascular endothelial growth factor
Disruption of the neurovascular unit following cerebral ischemia affects protetive function of the blood-brain barrier, thus contributing to vasogenic edema and hemorrhagic transformation.
This study explored the effects of mediators released from neurovascular unit cells on death of brain endothelial cells, astrocytes, pericytes, and microglia during oxygen glucose deprivation.
Rat primary cell cultures were exposed either to oxygen glucose deprivation or control conditions. Cell death and released angiogenic factors were assessed from media collected from cultures. For some experiments, astrocyte-conditioned media, pericyte-conditioned media, and microglia-conditioned media, collected from the corresponding cell culture after six-hour oxygen glucose deprivation, were added to the media during oxygen glucose deprivation incubations.
Brain endothelial cells were more susceptible to death following oxygen glucose deprivation than other neurovascular unit cells. Neither astrocyte-conditioned media nor vascular endothelial growth factor165 were protective for pericytes or brain endothelial cells during oxygen glucose deprivation. Vascular endothelial growth factor receptor antagonist significantly reduced cell death of brain endothelial cells treated with astrocyte-conditioned media or vascular endothelial growth factor165. Pericyte-conditioned media were protective for brain endothelial cells and microglia, but this was not mediated by pericyte-released angiopoietin 1. Soluble angiopoietin 1/angiopoietin 2 receptor Tie2 was protective for brain endothelial cells. Microglia-conditioned media were protective for astrocytes and brain endothelial cells, possibly through transforming growth factor β1 or interleukin 6.
Microglia-derived signaling molecules, but not angiogenic factors, were protective for neurovascular unit cells during oxygen glucose deprivation. This finding could identify a potential therapeutic target for ischemic stroke.