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imb12002-sup-0005-si.pdf63K

Table S1. Primers used for in situ hybridization, and primers and probes used for quantitative reverse-transcription PCR.

imb12002-sup-0001-si.tiff1148K

Figure S1. Reproducibility of the detection of Spots #3–5 in mushroom bodies (MBs) and optic lobes (OLs) of the worker brains by proteomic analysis. Eight sets of available data are shown for comparison of the intensities of Spots #3–5 between the MBs and OLs using two-dimensional electrophoresis. The panels (from right to left) show: Spot #3 in the MBs and OLs; Spots #4 and #5 in the MBs and OLs. Only small gel areas that contained Spot #3, and Spots #4 and #5 of the MBs, and the corresponding small gel areas in the OLs are shown. Spots #3–5 are indicated with arrows.

imb12002-sup-0002-si.tiff1148K

Figure S2. Reproducibility of the in situ hybridization analysis of reticulocalbin in the forager brain. Two different frontal sections of the forager brain hemisphere hybridized with a digoxigenin-labelled antisense probe for Apis mellifera reticulocalbin are shown. Scale bars = 100 μm.

imb12002-sup-0003-si.tiff1148K

Figure S3. Reproducibility of the in situ hybridization analysis of ryanodine receptor (ryr) in the forager brain. Three different frontal sections of a forager brain hemisphere hybridized with a digoxigenin-labelled antisense probe for Apis mellifera ryanodine receptor are shown. Scale bars = 100 μm.

imb12002-sup-0004-si.pdf252K

Figure S4. Quantitative reverse-transcription PCR (RT-PCR) of actin transcript. The amounts of actin transcript in each of seven forager mushroom body (MB) and optic lobe (OL) samples (each sample contained three to five individuals from the same colony) were determined using quantitative RT-PCR. The mean and standard error of seven samples were determined. The difference between the MB and OL was not significant (P > 0.05, paired t-test).

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