Mushroom body-preferential expression of proteins/genes involved in endoplasmic reticulum Ca2+-transport in the worker honeybee (Apis mellifera L.) brain
Article first published online: 21 NOV 2012
© 2012 Royal Entomological Society
Insect Molecular Biology
Volume 22, Issue 1, pages 52–61, February 2013
How to Cite
Uno, Y., Fujiyuki, T., Morioka, M. and Kubo, T. (2013), Mushroom body-preferential expression of proteins/genes involved in endoplasmic reticulum Ca2+-transport in the worker honeybee (Apis mellifera L.) brain. Insect Molecular Biology, 22: 52–61. doi: 10.1111/imb.12002
- Issue published online: 18 JAN 2013
- Article first published online: 21 NOV 2012
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan
- Japan Society for the Promotion of Science for young scientists
Table S1. Primers used for in situ hybridization, and primers and probes used for quantitative reverse-transcription PCR.
Figure S1. Reproducibility of the detection of Spots #3–5 in mushroom bodies (MBs) and optic lobes (OLs) of the worker brains by proteomic analysis. Eight sets of available data are shown for comparison of the intensities of Spots #3–5 between the MBs and OLs using two-dimensional electrophoresis. The panels (from right to left) show: Spot #3 in the MBs and OLs; Spots #4 and #5 in the MBs and OLs. Only small gel areas that contained Spot #3, and Spots #4 and #5 of the MBs, and the corresponding small gel areas in the OLs are shown. Spots #3–5 are indicated with arrows.
Figure S2. Reproducibility of the in situ hybridization analysis of reticulocalbin in the forager brain. Two different frontal sections of the forager brain hemisphere hybridized with a digoxigenin-labelled antisense probe for Apis mellifera reticulocalbin are shown. Scale bars = 100 μm.
Figure S3. Reproducibility of the in situ hybridization analysis of ryanodine receptor (ryr) in the forager brain. Three different frontal sections of a forager brain hemisphere hybridized with a digoxigenin-labelled antisense probe for Apis mellifera ryanodine receptor are shown. Scale bars = 100 μm.
Figure S4. Quantitative reverse-transcription PCR (RT-PCR) of actin transcript. The amounts of actin transcript in each of seven forager mushroom body (MB) and optic lobe (OL) samples (each sample contained three to five individuals from the same colony) were determined using quantitative RT-PCR. The mean and standard error of seven samples were determined. The difference between the MB and OL was not significant (P > 0.05, paired t-test).
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