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imb12005-sup-0001-FS1.pdf100K

Figure S1. Sexing individual pupae by detection of a male-specific dsx mRNA variant. RNA from individual 5-h male or female pupae was assayed for expression of a male-specific doublesex isoform by RT-PCR. The arrow indicates the male-specific doublesex cDNA band (1198 bp). Non-specific bands may appear in female samples as shown.

imb12005-sup-0002-FS2.pdf14K

Figure S2. Detection of upstream sequences from the Hex-lacZ reporter gene transcript in pupae. RNA from L4 larvae of the 2M2 line

imb12005-sup-0003-si.doc71K

Table S1. Primer pairs used to assess Adh-lacZ-SV40 mRNA expression by quantitative real-time PCR or reverse-transcription PCR.

Table S2. Mean ‘raw’ CT values for 5-hour pupae of 3 transgenic lines and the host, We.

Table S3. Mean ‘raw’ CT values for different developmental stages of the 2M2 line.

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