Targeting gene expression to the female larval fat body of transgenic Aedes aegypti mosquitoes
Article first published online: 13 DEC 2012
© 2012 Royal Entomological Society
Insect Molecular Biology
Volume 22, Issue 1, pages 18–30, February 2013
How to Cite
Totten, D. C., Vuong, M., Litvinova, O. V., Jinwal, U. K., Gulia-Nuss, M., Harrell, R. A. and Beneš, H. (2013), Targeting gene expression to the female larval fat body of transgenic Aedes aegypti mosquitoes. Insect Molecular Biology, 22: 18–30. doi: 10.1111/imb.12005
- Issue published online: 18 JAN 2013
- Article first published online: 13 DEC 2012
- National Institutes of Health, USA. Grant Number: AI046738
- College of Medicine Research Council
Figure S1. Sexing individual pupae by detection of a male-specific dsx mRNA variant. RNA from individual 5-h male or female pupae was assayed for expression of a male-specific doublesex isoform by RT-PCR. The arrow indicates the male-specific doublesex cDNA band (1198 bp). Non-specific bands may appear in female samples as shown.
Figure S2. Detection of upstream sequences from the Hex-lacZ reporter gene transcript in pupae. RNA from L4 larvae of the 2M2 line
Table S1. Primer pairs used to assess Adh-lacZ-SV40 mRNA expression by quantitative real-time PCR or reverse-transcription PCR.
Table S2. Mean ‘raw’ CT values for 5-hour pupae of 3 transgenic lines and the host, We.
Table S3. Mean ‘raw’ CT values for different developmental stages of the 2M2 line.
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