Transgene-mediated suppression of the RNA interference pathway in Aedes aegypti interferes with gene silencing and enhances Sindbis virus and dengue virus type 2 replication
Article first published online: 18 JAN 2013
© 2013 Royal Entomological Society
Insect Molecular Biology
Volume 22, Issue 1, pages 104–114, February 2013
How to Cite
Khoo, C. C. H., Doty, J. B., Heersink, M. S., Olson, K. E. and Franz, A. W. E. (2013), Transgene-mediated suppression of the RNA interference pathway in Aedes aegypti interferes with gene silencing and enhances Sindbis virus and dengue virus type 2 replication. Insect Molecular Biology, 22: 104–114. doi: 10.1111/imb.12008
- Issue published online: 18 JAN 2013
- Article first published online: 18 JAN 2013
- National Institutes of Health. Grant Number: AI073298-01
Figure S1. Intensity of SINV-TR339eGFP infection in Carb/B2-133 and HWE mosquitoes. Carb/B2-133 mosquitoes over-expressed FHV-B2 in midguts of blood-fed females from the Aedes aegypti carboxypeptidase A-1 (AeCPA-1) promoter. The transgenic line was unstable and was lost after this single challenge experiment. Females were orally challenged with SINV-TR339eGFP 1 week post-eclosion. Each data point represents the virus titre (pfu/ml) in an individual midgut or carcass as analysed by plaque assays at 7 days post-bloodmeal. P-values (α = 0.05) for infection rates and mean viral titres are shown in the table.
Figure S2. Survival curve of PUbB2 P61 and HWE control mosquitoes infected with SINV-TE3′2JeGFP. Seven days post-eclosion, PUbB2 P61 and HWE females were either fed with a non-infectious bloodmeal or with a bloodmeal containing SINV-TE3′2JeGFP (titre in the bloodmeal: 2.7 × 107 pfu/ml). After blood-feeding, 20 mosquitoes of each treatment were put into 470 ml cardboard containers and provided with sugar and water. For a period of 29 days after blood-feeding the daily number of surviving mosquitoes in each container was recorded. Relative survival (average of three replicates per treatment) is shown.
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