Funding: The authors gratefully acknowledge the Research Foundation of Flanders (FWO-Vlaanderen G041708N and GO62811N) and the University of Leuven Research Foundation (GOA 2010/14) for financial support. M.V.V. and D.C. are funded by the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Extending the honey bee venome with the antimicrobial peptide apidaecin and a protein resembling wasp antigen 5
Article first published online: 25 JAN 2013
© 2013 Royal Entomological Society
Insect Molecular Biology
Volume 22, Issue 2, pages 199–210, April 2013
How to Cite
Van Vaerenbergh, M., Cardoen, D., Formesyn, E. M., Brunain, M., Van Driessche, G., Blank, S., Spillner, E., Verleyen, P., Wenseleers, T., Schoofs, L., Devreese, B. and de Graaf, D. C. (2013), Extending the honey bee venome with the antimicrobial peptide apidaecin and a protein resembling wasp antigen 5. Insect Molecular Biology, 22: 199–210. doi: 10.1111/imb.12013
- Issue published online: 5 MAR 2013
- Article first published online: 25 JAN 2013
- Research Foundation of Flanders. Grant Numbers: G041708N, GO62811N
- University of Leuven Research Foundation. Grant Number: GOA 2010/14
- Institute for the Promotion of Innovation through Science and Technology in Flanders
Figure S1. Peptide mass fingerprint and tandem mass spectrometry fragmentation spectrum of the identified apidaecin peptide in venom gland tissue.
Figure S2. Nucleotide and protein sequence alignments of the cloned honey bee Ag5-like sequence with the National Center for Biotechnology Information prediction (by ClustalW).
Figure S3. Coomassie blue staining of sodium dodecyl sulphate polyacrylamide gel electrophoresis-separated synthetic apidaecin peptide and purified recombinants uterocalin, Ves v 5 and honey bee Ag5-like protein.
Table S1. Information about beekeeper sera.
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