Supporting information SI1. Sperm-mediated or microinjected plasmid transfer into honey bee embryos: pilot experiments.

Supporting information SI2. Blastp search of D. melanogaster Elav protein amino acid sequence against the honey bee protein database at the National Center for Biotechnology Information.

Supporting information SI3. Sequences with the designated promoter regions.

Supporting information SI4. The mean numbers of Sf21-cells and fluorescent Sf21-cells per picture that were used to study the promoter activity. The numbers were estimated from pictures taken from 10 transfections for each promoter construct.

Supporting information SI5. The mean numbers of Sf21-cells per picture detected in pictures that were used to study the impact of temperature shifts.

Supporting information SI6. Oligonucleotides that were used in PCRs.

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