Noncanonical expression of caudal during early embryogenesis in the pea aphid Acyrthosiphon pisum: maternal cad-driven posterior development is not conserved
Article first published online: 20 MAY 2013
© 2013 Royal Entomological Society
Insect Molecular Biology
Volume 22, Issue 4, pages 442–455, August 2013
How to Cite
Chang, C.-c., Hsiao, Y.-m., Huang, T.-Y., Cook, C. E., Shigenobu, S. and Chang, T.-H. (2013), Noncanonical expression of caudal during early embryogenesis in the pea aphid Acyrthosiphon pisum: maternal cad-driven posterior development is not conserved. Insect Molecular Biology, 22: 442–455. doi: 10.1111/imb.12035
- Issue published online: 2 JUL 2013
- Article first published online: 20 MAY 2013
- National Science Council. Grant Numbers: 101–2313-B-002–059-MY3, 101–2321-B-002–090-MY2, 8–2311-565-B-001–015-MY3, 101–2311-B-001–005
- Academia Sinica
- BAPHIQ of the Agricultural Council. Grant Number: 188.8.131.52-B3(4)
- National Taiwan University. Grant Numbers: NTU-CESRP 101R4602D3, NTU-CESRP 102R7602D3
Figure S1. Developmental expression of ApHb protein during oogenesis and early embryogenesis in the asexual pea aphid. Germaria and anterior region of egg chambers are to the left. Immunostaining protocols were as described by Chang et al. (2006). (A) Ovarioles stained with affinity-purified antibody against ApHb protein. Staining signals of ApHb were identified in germaria and cytoplasm of embryos undergoing nuclear division (here we present staining results in stage-3 embryos as an example). Intensity of ApHb signals declined in embryos undergoing blastulation (stages 5–6 of development). Arrowhead and hollow arrowhead indicate places where localization of Aphb mRNA was shown in stage-3 and stage-6 embryos in Fig. 5A, respectively. (B) Negative control. Ovarioles were stained without the application of primary antibody. No staining signals were detected. For abbreviations please refer the legend of Fig. 2. Scale bar, 20 μm.
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