Role of a gamma-aminobutryic acid (GABA) receptor mutation in the evolution and spread of Diabrotica virgifera virgifera resistance to cyclodiene insecticides
Article first published online: 11 JUL 2013
© 2013 Royal Entomological Society
Insect Molecular Biology
Volume 22, Issue 5, pages 473–484, October 2013
How to Cite
Wang, H., Coates, B. S., Chen, H., Sappington, T. W., Guillemaud, T. and Siegfried, B. D. (2013), Role of a gamma-aminobutryic acid (GABA) receptor mutation in the evolution and spread of Diabrotica virgifera virgifera resistance to cyclodiene insecticides. Insect Molecular Biology, 22: 473–484. doi: 10.1111/imb.12037
- Issue published online: 3 SEP 2013
- Article first published online: 11 JUL 2013
- Biodiversité program of the French Agence Nationale de la Recherche. Grant Number: ANR-06-BDIV-008-01
- United States Department of Agriculture, Agricultural Research Service. Grant Number: 3625–22000-017-00D
Figure S1. Alignment of the deduced amino acid sequence of the Diabrotica virgifera virgifera gamma-aminobutyric acid (GABA) receptor gene from the susceptible non-diapause strain with GABA receptor isoform b from Tribolium castaneum. An asterisk above sequence indicates position of the alanine (A) to Serine/Glycine (S/G) substitution observed in cyclodiene-resistant species (ffrench-Constant et al., 2000). Transmembrane domains (TM1-TM3) are boxed.
Figure S2. Restriction fragment length polymorphism of D. v. virgifera BAC clones that encode the gamma-aminobutyric acid (GABA) receptor marker. L = Lambda EcoRI + HindIII ladder; lane 1: clone 47B13, lane 2: clone 82A13, lane 3: clone 129I06, lane 4 clone 178L17, lane 5, clone 222A09; lane 6 clone 236O22; lane 7, clone 248K09; lane 8, clone 269I11; lane 9, clone 274K02.
Table S1. BlastX search results of the National Center for Biotechnology Information nonredundant protein database queried by the Diabrotica virgifera virgifera gamma-aminobutyric acid (GABA) receptor gene. Percent identities are to the deduced D. v. virgifera amino acid sequence.
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