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imb12049-sup-0001-si.tif1171K

Figure S1. Multiple sequence alignment of 55 basic helix-loop-helix (bHLH) motifs from Anopheles gambiae. The scheme at the top illustrates the locations and boundaries of the basic, helix 1, loop and helix 2 regions within the bHLH domain following Ferre-D'Amare et al. (1993). Identical amino acid residues are shaded in black, conserved ones in dark grey and less conserved ones in light grey. Sites with highly conserved amino acid residues are marked with asterisks on the top.

imb12049-sup-0002-si.tif1209K

Figure S2. Multiple sequence alignment of 57 basic helix-loop-helix (bHLH) motifs from Culex quinquefasciatus. The scheme at the top illustrates the locations and boundaries of the basic, helix 1, loop and helix 2 regions within the bHLH domain following Ferre-D'Amare et al. (1993). Identical amino acid residues are shaded in black, conserved ones in dark grey and less conserved ones in light grey. Sites with highly conserved amino acid residues are marked with asterisks on the top.

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Table S1. A complete list of basic helix-loop-helix (bHLH) proteins encoded in the genome of Anopheles gambiae str. PEST.

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Table S2. A complete list of basic helix-loop-helix (bHLH) proteins encoded in the genome of Culex quinquefasciatus.

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Table S3. Coding regions, intron analysis and expressed sequence tag (EST) evidence of 55 Aedes aegypti basic helix-loop-helix (AabHLH) motifs.

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Table S4. Coding regions, intron analysis and expressed sequence tag (EST) evidence of 55 Anopheles gambiae basic helix-loop-helix (AgbHLH) motifs.

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Table S5. Coding regions, intron analysis and expressed sequence tag (EST) evidence of 57 Culex quinquefasciatus basic helix-loop-helix (CqbHLH) motifs.

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Table S6. The basic helix-loop-helix (bHLH) family members in nine insect species.

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Table S7. Evolutionary rates of mosquito basic helix-loop-helix (bHLH) genes.

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Appendix S1. Analytical data of the intron sequence from the coding region of the Aedes aegypti cropped (AaCrp) motif.

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