Genome-wide screening for components of small interfering RNA (siRNA) and micro-RNA (miRNA) pathways in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
Article first published online: 13 AUG 2013
© 2013 Royal Entomological Society
Insect Molecular Biology
Volume 22, Issue 6, pages 635–647, December 2013
How to Cite
Xu, H.-J., Chen, T., Ma, X.-F., Xue, J., Pan, P.-L., Zhang, X.-C., Cheng, J.-A. and Zhang, C.-X. (2013), Genome-wide screening for components of small interfering RNA (siRNA) and micro-RNA (miRNA) pathways in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae). Insect Molecular Biology, 22: 635–647. doi: 10.1111/imb.12051
- Issue published online: 5 NOV 2013
- Article first published online: 13 AUG 2013
- National Basic Research Program of China. Grant Number: 2010CB126205
- National Science Foundation of China. Grant Number: 31201509
Figure S1. The mortality of BPH with dsDcr1 and dsAgo1 treatment. The third instar nymphs were injected with dsRNA at dose of 50 ng each nymph. At 4 days after injection, the relative transcripts of target genes were examined by quantitative real-time PCR (A). Mortality was calculated every two days (B). The third instar nymphs were injected either with dsGfp, PBS, or non-injection (Wt), and mortality was monitored every 2 days. Standard bars represent three independent repeats. Significant difference was examined by Tukey test (P < 0.05).
Table S1. Core RNAi components in BPH.
Table S2. Candidate genes for dsRNA uptake in BPH.
Table S3. Primers used in this study.
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