Identification of taste receptors and proteomic characterization of the antenna and legs of Tribolium brevicornis, a stored food product pest

Authors

  • T. Alabi,

    Corresponding author
    1. Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium
    2. National Polytechnic Institute FHB, ESA of Yamoussoukro, Yamoussoukro, Ivory Cost
    • Correspondence: Taofic Alabi, Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liege, Passage des Déportés, 2 B-5030 Gembloux, Belgium. Tel.: +32 81622287; fax: +32 81622312; e-mail: entomologie.gembloux@ulg.ac.be

    Search for more papers by this author
  • F. Marion-Poll,

    1. AgroParisTech, Département Sciences de la Vie et Santé, Paris, France
    2. INRA, UMR1272 Physiologie de l'Insecte: Signalisation et Communication, Versailles, France
    3. Université Pierre et Marie Curie, UMR1272 Physiologie de l'Insecte: Signalisation et Communication, Versailles, France
    Search for more papers by this author
  • M. Danho,

    1. National Polytechnic Institute FHB, ESA of Yamoussoukro, Yamoussoukro, Ivory Cost
    Search for more papers by this author
  • G. D. Mazzucchelli,

    1. Mass Spectrometry Laboratory, University of Liege, Liege, Belgium
    Search for more papers by this author
  • E. De Pauw,

    1. Mass Spectrometry Laboratory, University of Liege, Liege, Belgium
    Search for more papers by this author
  • E. Haubruge,

    1. Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium
    Search for more papers by this author
  • F. Francis

    1. Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium
    Search for more papers by this author

Abstract

Chemoreception plays an important role in mediating a diverse range of behaviours, including predation and food selection. In the present study, we combined anatomical observations, electrophysiology and proteomics to investigate sensilla that mediate chemoreception on the antenna and the legs of Tribolium. Scanning electron microscopy was used to differentiate the coxal and trochanteral segments of the pro-, meso- and metathoracic legs by the presence of sensilla trichoidea and chaetica, while the antennae were covered with five types of sensilla (chaetica, basiconica, trichoidea, squamiformia and coeloconica). Antenna morphology and ultrastructure were similar in both sexes. Electrophysiological recordings allowed us to characterize a row of small sensilla basiconica on the terminal segment of the antenna as taste receptors, responding to sucrose and NaCl. Proteomics investigations of antennae and legs yielded several proteins with specific interest for those involved in chemoreception. Odorant-binding proteins were antenna-specific, while chemosensory proteins were detected in both tissues.

Ancillary