Figure S1. Alignments of the nucleotide sequences of Gryllus fne 5′ rapid amplification of cDNA ends (RACE) clones. (A) The 10-nucleotide sequence of 5′ RACE clones corresponding to the 5′ UTR of Gryllus fne-A isoform are aligned. (B) The 3-nucleotide sequence of 5′ RACE clones corresponding to the 5′ UTR of Gryllus fne-B isoform are aligned. Nucleotide substitutions are indicated as white text on a black background. The consensus coding sequences and their deduced protein sequences are indicated by grey-shaded boxes. The consensus nucleotide sequences were registered to GenBank.


Figure S2. Stage-dependent expression patterns of three house-keeping genes (Gryllus β-actin, Ef1alpha and rp49) during embryonic development. The cDNA fragments corresponding to of Gryllus β-actin, Ef1alpha and rp49 genes were amplified by RT-PCR from cricket embryos. PCR products were run on a 1.5% agarose gel and stained with ethidium bromide. Three house-keeping genes were stably expressed during embryonic development.

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