Tissue-specific promoter usage and diverse splicing variants of found in neurons, an ancestral Hu/ELAV-like RNA-binding protein gene of insects, in the direct-developing insect Gryllus bimaculatus
Article first published online: 1 NOV 2013
© 2013 The Royal Entomological Society
Insect Molecular Biology
Volume 23, Issue 1, pages 26–41, February 2014
How to Cite
Watanabe, T. and Aonuma, H. (2014), Tissue-specific promoter usage and diverse splicing variants of found in neurons, an ancestral Hu/ELAV-like RNA-binding protein gene of insects, in the direct-developing insect Gryllus bimaculatus. Insect Molecular Biology, 23: 26–41. doi: 10.1111/imb.12057
- Issue published online: 1 JAN 2014
- Article first published online: 1 NOV 2013
- JSPS. Grant Numbers: 23300113, 24●3065
Figure S1. Alignments of the nucleotide sequences of Gryllus fne 5′ rapid amplification of cDNA ends (RACE) clones. (A) The 10-nucleotide sequence of 5′ RACE clones corresponding to the 5′ UTR of Gryllus fne-A isoform are aligned. (B) The 3-nucleotide sequence of 5′ RACE clones corresponding to the 5′ UTR of Gryllus fne-B isoform are aligned. Nucleotide substitutions are indicated as white text on a black background. The consensus coding sequences and their deduced protein sequences are indicated by grey-shaded boxes. The consensus nucleotide sequences were registered to GenBank.
Figure S2. Stage-dependent expression patterns of three house-keeping genes (Gryllus β-actin, Ef1alpha and rp49) during embryonic development. The cDNA fragments corresponding to of Gryllus β-actin, Ef1alpha and rp49 genes were amplified by RT-PCR from cricket embryos. PCR products were run on a 1.5% agarose gel and stained with ethidium bromide. Three house-keeping genes were stably expressed during embryonic development.
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