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imb12067-sup-0001-fs1.pdf398K

Figure S1. Gene ontology (GO) assignments for the Meligethes aeneus transcriptome. GO-Slim assignments predicted for their involvement in (A) molecular functions and (B) biological processes. All data are presented at level 3 GO categorization. Classified gene objects are shown as absolute numbers. Note that one gene object can be classified into more than one class, therefore, the total number of gene objects is not identical to the number of contigs with GO associations.

imb12067-sup-0002-fs2.pdf459K

Figure S2. Differential gene ontology (GO) term representation between Meligethes aeneus adults and larvae. Differences are shown as the percentage of sequences associated with a specific GO category in the test set (total number of differentially expressed contigs between adults and larvae) vs the reference set (transcriptome backbone assembly) using a Fisher's exact test implemented Blast2GO-PRO.

imb12067-sup-0003-fs3.pdf2113K

Figure S3. C-type lysozyme alignment. Amino acid alignment of predicted C-type lysozymes from Meligethes aeneus together with predicted protein sequences from publicly-available insect, invertebrate and human sequence datasets. Amino acid sequences were aligned using muscle and visualized in clc genomics workbench v6.0.2. Identical or similar amino acids are shaded in the same colour. Abbreviations: Artogeia rapae (Arap), Antheraea mylitta (Amyl), Bombyx mori (Bmor), Drosophila melanogaster (Dmel), Glossina morsitans morsitans (Gmmo), Harmonia axyridis (Haxy), Heliothis virescens (Hvir), Homo sapiens (Hsap), Meligethes aeneus (Maen), Manduca sexta (Msex), Simulium vittatum (Svit), Trichoplusia ni (Tni), Tribolium castaneum (Tcas).

imb12067-sup-0004-fs4.pdf2713K

Figure S4. I-type lysozyme alignment. Amino acid alignment of predicted I-type lysozyme from Meligethes aeneus together with predicted protein sequences from publicly-available insect, invertebrate and human sequence datasets. Amino acid sequences were aligned using muscle and visualized in clc genomics workbench v6.0.2. Identical or similar amino acids are shaded in the same color. Abbreviations: Anopheles gambiae (Agam), Aedes aegypti (Aaeg), Bombyx mori (Bmor), Culex quincefasciatus (Cqui), Drosophila melanogaster (Dmel), Eisenia andrei (Eand), Galleria mellonella (Gmel), Harmonia axyridis (Haxy), Hirudo medicinalis (Hmed), Ixodes scapularis (Isca), Meligethes aeneus (Maen), Pediculus humanus corporis (Phum), Papilio xuthus (Pxut), Strongylocentrotus purpuratus (Spur), Simulium vittatum (Svit), Sitophilus zeamais (Szea),

imb12067-sup-0005-files1.xlsx20671K

File S1. Complete annotation file of the assembled pollen beetle RNA-Seq data. The list shows Meligethes aeneus contig IDs, sequence length, contig sequences, top Blast hits (if any) in the NCBI nr database for each unique contig, including accession number, E-value and percentage similarity. The gene ontology (GO) terms were obtained from Blast2GO.

imb12067-sup-0006-files2.xlsx475K

File S2. Differentially expressed genes between larvae and adult control beetles. The list shows absolute numbers (mapped reads), relative normalized values (RPKM), calculated fold-change differences and annotation for all contigs displaying eightfold or higher differential expression.

imb12067-sup-0007-files3.xlsx346K

File S3. Differentially expressed genes in immune-challenged adult beetles. The list shows absolute numbers (mapped reads), relative normalized values (RPKM), calculated fold-change differences and annotation for all contigs displaying fourfold or higher differential expression.

imb12067-sup-0008-ts1.doc144K

Table S1. List of immunity-related genes identified in the pollen beetle transcriptome. The list shows Meligethes aeneus immunity-related genes, relative expression levels and fold-change differences between samples based on the mapping of individual reads against the backbone assembly.

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