Constructing the major biosynthesis pathways for amino acids in the brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae), based on the transcriptome data
Article first published online: 13 DEC 2013
© 2013 The Royal Entomological Society
Insect Molecular Biology
Volume 23, Issue 2, pages 152–164, April 2014
How to Cite
Wan, P.-J., Yang, L., Wang, W.-X., Fan, J.-M., Fu, Q. and Li, G.-Q. (2014), Constructing the major biosynthesis pathways for amino acids in the brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae), based on the transcriptome data. Insect Molecular Biology, 23: 152–164. doi: 10.1111/imb.12069
- Issue published online: 6 MAR 2014
- Article first published online: 13 DEC 2013
- National Basic Research Program of China. Grant Number: 2010CB126200
- National Natural Science Foundation of China. Grant Numbers: 31371939, 30370937
Figure S1. Multiple sequence alignment of NlYDHAD and dihydroxy-acid dehydratases of several yeast species. The amino acid sequence N. lugens (NlDHAD) is aligned with the proteins from Aspergillus niger CBS 513.88 (AnDHAD, XP_001390333), Neosartorya fischeri NRRL 181 (NfDHAD, XP_001266525) and Penicillium marneffei ATCC 18224 (PmDHAD, XP_002143860).
Figure S2. Multiple sequence alignment of NlYBCAT and branched-chain amino acid transaminase (BCAT) of several yeast species. The amino acid sequence N. lugens (NlBCAT) is aligned with the proteins from Aspergillus flavus NRRL3357 (AfBCAT, XP_002377249.1) and Saccharomyces cerevisiae S288c (ScBAT1 (AAB69733) and ScBAT2 (NP_012682)). The lysine residue identified as the active site of the Escherichia coli enzyme is highlighted in italics. _, * and # showed substrate-cofactor binding pocket, catalytic residue and homodimer interface on conserved domain BCAT beta family. See legend to Table 1 for further explanation.
Table S1. Counts of enzymes identified among 521 sequences
Table S2. Primer sequences and sizes of PCR production for reverse transcription (RT)-PCR, RACE and tissue-biased expression test in N. lugens
Table S3. Relative synonymous codon usage values of the genes for 493 putative enzymes in the synthesis of protein amino acids
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