An analysis of the small RNA transcriptome of four developmental stages of the citrus red mite (Panonychus citri)
Article first published online: 11 DEC 2013
© 2013 The Royal Entomological Society
Insect Molecular Biology
Volume 23, Issue 2, pages 216–229, April 2014
How to Cite
Liu, B., Dou, W., Ding, T.-B., Zhong, R., Liao, C.-Y., Xia, W.-K. and Wang, J.-J. (2014), An analysis of the small RNA transcriptome of four developmental stages of the citrus red mite (Panonychus citri). Insect Molecular Biology, 23: 216–229. doi: 10.1111/imb.12075
- Issue published online: 6 MAR 2014
- Article first published online: 11 DEC 2013
- Special Fund for Agro-scientific Research in the Public Interest. Grant Number: 201103020
- Program for Innovative Research Team in Universities. Grant Number: IRT0976
- National Natural Science Foundation. Grant Number: 31171851
- Modern Agro-industry (Citrus) Technology Research System of China
- Fundamental Research Funds for the Central Universities. Grant Number: XDJK2013A017
Figure S1. Analysis of nucleotide bias of the novel microRNA (miRNA) candidates in the four development stages of P. citri. A: the nucleotide bias analysis at each position of novel miRNA candidates in the embryo, larva, nymph and adult (from top to bottom), B: the analysis of first nucleotide bias of novel RNAs (20–24 bp in length). The colours dull-red, green, red and blue represent guanine (G), cytosine (C), adenine (A) and uracil (U), respectively.
Table S1. Summary of data cleaning in the four sequencing libraries.
Table S2. The statistics of total clean small RNAs mapped to chromosomes of Tetranychus urticae in the four libraries.
Table S3. The known microRNAs and their expression level in embryo, larva, nymph and adult.
Table S4. Known microRNAs in Panonychus citri.
Table S5. Known microRNA families in the four developmental stages.
Table S6. The pairwise expression level comparison results of the known microRNAs in larva and embryo.
Table S7. The novel microRNAs and their precursors in all four libraries.
Table S8. Target gene prediction for novel miRNAs (continued).
Table S9. The enriched Gene Ontology (P < 0.05) terms of predicted novel microRNA target genes in the four libraries.
Table S10. The enriched pathways (Q < 0.05) of predicted novel microRNA target genes in the four libraries.
Table S11. The small RNA-associated repeat sequence types and their expression in embryo, larva, nymph and adult.
Table S12. The 24–25-bp long small RNAs mapped to genome.
Table S13. Primers used for reverse transcription (RT)-PCR, and quantitative real-time PCR.
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