Scavenger receptor-mediated endocytosis facilitates RNA interference in the desert locust, Schistocerca gregaria

Authors

  • N. Wynant,

    Corresponding author
    1. Molecular Developmental Physiology and Signal Transduction, Department of Animal Physiology and Neurobiology, KU Leuven, Leuven, Belgium
    • Correspondence: Niels Wynant, Molecular Developmental Physiology and Signal Transduction, Department of Animal Physiology and Neurobiology, KU Leuven, Naamsestraat 59, P.O. Box 02465, B-3000 Leuven, Belgium. Tel.: +32 16323900; fax: +32 16323902; e-mail: niels.wynant@bio.kuleuven.be

    Search for more papers by this author
  • D. Santos,

    1. Molecular Developmental Physiology and Signal Transduction, Department of Animal Physiology and Neurobiology, KU Leuven, Leuven, Belgium
    Search for more papers by this author
  • P. Van Wielendaele,

    1. Molecular Developmental Physiology and Signal Transduction, Department of Animal Physiology and Neurobiology, KU Leuven, Leuven, Belgium
    Search for more papers by this author
  • J. Vanden Broeck

    1. Molecular Developmental Physiology and Signal Transduction, Department of Animal Physiology and Neurobiology, KU Leuven, Leuven, Belgium
    Search for more papers by this author

Abstract

RNA interference (RNAi) has become a widely used loss-of-function tool in eukaryotes; however, the delivery of double-stranded (ds)RNA) to the target cells remains a major challenge when exploiting the RNAi-technology. In insects, the efficiency of RNAi is highly species-dependent. Yet, the mechanism of cell entry in insects has only been characterized in a cell line of the fruit fly, Drosophila melanogaster, a species that is well known to be poorly amenable to environmental RNAi. In the present paper, we demonstrate that silencing vacuolar H-ATPase 16 (vha16) and clathrin heavy chain (clath), two components of the Clathrin-dependent endocytosis pathway, together with pharmacological inhibition of scavenger receptors with polyinosine and dextran sulphate, can significantly attenuate the highly robust RNAi response in the desert locust, Schistocerca gregaria.

Ancillary