Figure S1. Fractions of CD4+ and CD8+ subsets in the spleen.

Figure S2. In vitro proliferation of wild-type (WT) and transglutaminase 2 deficient (TG2−/−) T cells. Splenic T cells were isolated, loaded with CFSE, and stimulated with anti-CD3 and anti-CD28 antibodies for 6 days.

Figure S3. Naive T cells from un-immunized mice were isolated, stained for CD8, CD44, CD62L and CD127 without stimulation, gated on CD8, and subjected to flow cytometric analysis.

Figure S4. Comparison of the dead cell frequencies between wild-type (WT) and transglutaminase 2 deficient (TG2−/−) T cells after 3-day in vitro activation with anti-CD3 and anti-CD28 antibodies, as shown in Fig. 3.

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