Notch1 is an important mediator for enhancing of B-cell activation and antibody secretion by Notch ligand

Authors

  • Jung-Ah Kang,

    1. School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Korea
    2. Bio Imaging Research Centre and Immune Synapse Research Centre, Gwangju Institute of Science and Technology (GIST), Gwangju, Korea
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  • Woo-Seok Kim,

    1. School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Korea
    2. Bio Imaging Research Centre and Immune Synapse Research Centre, Gwangju Institute of Science and Technology (GIST), Gwangju, Korea
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  • Sung-Gyoo Park

    Corresponding author
    1. School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Korea
    2. Bio Imaging Research Centre and Immune Synapse Research Centre, Gwangju Institute of Science and Technology (GIST), Gwangju, Korea
    • Correspondence: Dr Sung-Gyoo Park, School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, Korea.

      Email: sgpark@gist.ac.kr

      Senior author: Sung-Gyoo Park

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Summary

The roles of Notch1 and Notch2 in T-cell function have been well studied, but the functional roles of Notch in B cells have not been extensively investigated, except for Notch2 involvement in peripheral marginal zone B-cell differentiation. This study examined the roles of Notch1 in murine primary B cells. During B-cell activation by B-cell receptor ligation, Notch1 was up-regulated while Notch2 was not. In addition, Notch1 up-regulation itself did not contribute to the further activation of B cells, but the Notch ligand was important for Notch1-mediated further B-cell activation. Moreover, Notch1 deficiency significantly decreased B-cell activation and antibody secretion under the presence of Notch ligand. These data suggest that Notch1 is an important mediator for enhancing B-cell activation and antibody secretion by Notch ligand.

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