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Data S1. This supplementary information contains particle size and SDS-PAGE analysis from the various antigen-adjuvant mixtures. In addition, this supplementary information contains data obtained from multiplex cytokine bead array as well as additional data regarding antibody endpoint titers, antibody-secreting plasma cells, and IL-5 cytokine production.

Figure S1. Particle size of antigen-adjuvant mixtures at 0, 4, or 24 hours after mixing and storage at 5 °C or room temperature.

Figure S2. Gold-stained blots of SDS-PAGE of antigen-adjuvant mixtures at 0, 4, or 24 hours after mixing and storage at 5 °C or room temperature.

Figure S3. Antigen-specific cytokine levels determined from multiplex cytokine bead assay using rH5-stimulated splenocyte supernatants.

Figure S4. Antibody responses to rH5 vaccines at different doses of rH5 with various emulsions as measured after prime and boost immunizations.

Figure S5. Antigen-specific IL-5 producing cells detected 4 weeks after boost immunization with rH5 vaccines at different doses of rH5 with various emulsions.

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