Factors influencing the assessment of lung function in mice with influenza-induced lung disease
Article first published online: 8 NOV 2012
© 2012 John Wiley & Sons Ltd
Influenza and Other Respiratory Viruses
Volume 7, Issue 6, pages 889–894, November 2013
How to Cite
Larcombe, A. N., Zosky, G. R., Thamrin, C., Bozanich, E. M., Hantos, Z. and Sly, P. D. (2013), Factors influencing the assessment of lung function in mice with influenza-induced lung disease. Influenza and Other Respiratory Viruses, 7: 889–894. doi: 10.1111/irv.12034
- Issue published online: 5 NOV 2013
- Article first published online: 8 NOV 2012
- Accepted 25 September 2012. Published Online 7 November 2012.
- NHMRC. Grant Numbers: #458561, #458562
- Hungarian Scientific Research. Grant Numbers: T42971, K67700
- Constant-phase model;
- forced oscillation technique;
- severe lung disease
Please cite this paper as: Larcombe et al. (2012) Factors influencing the assessment of lung function in mice with influenza-induced lung disease. Influenza and Other Respiratory Viruses DOI: 10.1111/irv.12034.
Background The constant-phase model (CPM) is commonly fit to respiratory system input impedance (Zrs) to estimate lung mechanics. Driving signal frequencies and the method of model fitting may influence the results, especially in cases of severe lung disease or under severe bronchoconstriction.
Objective To illustrate the effects of different CPM fits to Zrs data using a mouse model of influenza-induced lung disease.
Methods BALB/c mice infected with influenza (or control) were challenged with methacholine. The CPM was fitted to Zrs, measured between 0·25 and 19·625 Hz, using both unweighted and weighted fits. The effect of different lowest frequencies was assessed.
Results and Conclusions For influenza-infected mice, the unweighted fit was poor, and airway resistance (Raw) was often biologically impossible. The weighted fit provided more realistic estimates of Raw. Different model fits and minimal frequencies had little effect on tissue mechanics.