• Open Access

Retrospective serological survey of influenza viruses in backyard pigs from Mexico City

Authors


Correspondence: Dr. Humberto Ramírez-Mendoza, Departamento de Microbiología e Inmunología FMVZ-UNAM. Av. Universidad No. 3000. Copilco, Del. Coyoacán, CP 04510 Distrito Federal, México. Email: betosram@yahoo.es

Abstract

Background

In the present study, we analyzed the presence of antibodies to four different influenza viruses (pH1N1, hH1N1, swH1N1, and swH3N2) in the sera of 2094 backyard pigs from Mexico City. The sera were obtained between 2000 and 2009.

Objectives

The aim of this study was to perform a retrospective analysis of the 2000–2009 period to determine the seroprevalence of antibodies against pH1N1, hH1N1, swH1N1, and swH3N2 viruses in sera obtained from backyard pigs in Mexico City.

Methods

Antibody detection was conducted with hemagglutination inhibition assay (HI) using four influenza viruses. We used linear regression to analyze the tendency of antibody serum titers throughout the aforementioned span.

Results

We observed that the antibody titers for the pH1N1, swH1N1, and swH3N2 viruses tended to diminish over the study period, whereas the antibodies to hH1N1 remained at low prevalence for the duration of the years analyzed in this study. A non-significant correlation (P > 0·05) between antibody titers for pH1N1 and swH1N1 viruses was observed (0·04). It contrasts with the significance of the correlation (0·43) observed between the swH1N1 and swH3N2 viruses (P < 0·01).

Conclusions

Our findings showed no cross-antigenicity in the antibody response against the same subtype. Antibodies against pH1N1 virus were observed throughout the 10-year study span, implying that annual strains shared some common features with the pH1N1 virus since 2000, which would then be capable of supporting the ongoing presence of these antibodies.

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