• Open Access

Oxygen free radical involvement in acute lung injury induced by H5N1 virus in mice

Authors

  • Guimei He,

    1. Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing, China
    2. Institute of Molecular Ecology and Evolution, Institutes for Advanced Interdisciplinary Research, East China Normal University, Shanghai, China
    Search for more papers by this author
    • These authors contributed equally to this study.
  • Changgui Dong,

    1. Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing, China
    Search for more papers by this author
    • These authors contributed equally to this study.
  • Zhihua Luan,

    1. Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing, China
    2. Panjin Animal Disease Control Center, Liaoning, China
    Search for more papers by this author
    • These authors contributed equally to this study.
  • Bronwyn M. McAllan,

    1. School of Medical Sciences, Discipline of Physiology, Medical Foundation Building (K25), The University of Sydney, Sydney, NSW, Australia
    Search for more papers by this author
  • Tong Xu,

    1. Department of Veterinary Medicine, College of Animal Science, HeBei North University, Hebei, China
    Search for more papers by this author
  • Lihong Zhao,

    1. Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing, China
    Search for more papers by this author
  • Jian Qiao

    Corresponding author
    1. Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing, China
    • Correspondence: Jian Qiao, Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China.

      E-mail: qiaojian@cau.edu.cn

    Search for more papers by this author

  • The work has been carried out at China Agricultural University.

Abstract

Background

Acute lung injury is an important cause of death in humans infected with H5N1. It has been found that oxygen free radicals (OFRs) are elevated in lung tissue during influenza virus infections. In this study, we used a mouse model to explore the role of OFRs in acute lung injury caused by H5N1 viral infection.

Methods

Four- to six-week-old male specific pathogen-free BALB/c mice were inoculated intranasally with 105 50% tissue culture infective doses (TCID50) of highly pathogenic A/Chicken/Hebei/108/2002 (H5N1) viruses and were then given 1000 IU of lauric acid modified superoxide dismutase (LA-SOD) by intraperitoneal injection, starting 2 days post-infection and continuing for 6 days.

Results

The extent of lung injury and the concentration of OFRs were higher, and the SOD activity was lower in H5N1 virus-infected mice than that in uninfected control mice on days 3, 6, and 7 post-inoculation. Weak amelioration of clinical signs, a minor decrease in the total mortality and the extent of lung injury, and the lower OFRs concentration were seen in the LA-SOD treatment group, but a reduction in lung virus titers was not observed in the LA-SOD treatment at all time points.

Conclusions

The LA-SOD treatment has a mild inhibitory effect on H5N1 influenza virus infection in mice. OFRs, therefore, might play an important role in the pathogenesis of acute lung injury induced by H5N1 virus.

Ancillary