Influenza A virus survival in water is influenced by the origin species of the host cell

Authors

  • Sayuri Shigematsu,

    1. Institut Pasteur, Environment and Infectious risks Unit, Laboratory for Urgent Response to Biological Threats (CIBU), Paris, France
    2. Nagasaki University Graduate School of Biomedical Science, Division of Cytokine Signaling, Department of Molecular Microbiology and Immunology, Nagasaki, Japan
    Search for more papers by this author
  • Amélie Dublineau,

    1. Institut Pasteur, Environment and Infectious risks Unit, Laboratory for Urgent Response to Biological Threats (CIBU), Paris, France
    Search for more papers by this author
    • These authors contributed equally to this work.
  • Olivier Sawoo,

    1. Institut Pasteur, Environment and Infectious risks Unit, Laboratory for Urgent Response to Biological Threats (CIBU), Paris, France
    2. University of Paris Diderot, Sorbonne Paris Cité (Cellule Pasteur), Paris, France
    Search for more papers by this author
    • These authors contributed equally to this work.
  • Christophe Batéjat,

    1. Institut Pasteur, Environment and Infectious risks Unit, Laboratory for Urgent Response to Biological Threats (CIBU), Paris, France
    Search for more papers by this author
  • Toshifumi Matsuyama,

    1. Nagasaki University Graduate School of Biomedical Science, Division of Cytokine Signaling, Department of Molecular Microbiology and Immunology, Nagasaki, Japan
    Search for more papers by this author
  • India Leclercq,

    Corresponding author
    1. Institut Pasteur, Environment and Infectious risks Unit, Laboratory for Urgent Response to Biological Threats (CIBU), Paris, France
    2. University of Paris Diderot, Sorbonne Paris Cité (Cellule Pasteur), Paris, France
    • Correspondence: India Leclercq, Institut Pasteur, Environment and Infectious risks Unit, Laboratory for Urgent Response to Biological Threats (CIBU), rue du Dr Roux, Paris, France.

      E-mail: india.leclercq@pasteur.fr

    Search for more papers by this author
  • Jean-Claude Manuguerra

    1. Institut Pasteur, Environment and Infectious risks Unit, Laboratory for Urgent Response to Biological Threats (CIBU), Paris, France
    Search for more papers by this author

Abstract

Background

Influenza A viruses have an envelope made of a lipid bilayer and two surface glycoproteins, the hemagglutinin and the neuraminidase. The structure of the virus is directly dependent on the genetic makeup of the viral genome except the glycosylation moieties and the composition of the lipid bilayer. They both depend on the host cell and are in direct contact with the environment, such as air or water. Virus survival is important for virus transmission from contaminated waters in the case of wild aquatic birds or from contaminated surface or air for humans.

Objective

The objective of this study was to check whether the origin species of the host cell has an influence on influenza A virus survival.

Method

The persistence in water at 35°C of viruses grown on either mammalian cells or avian cells and belonging to two different subtypes H1N1 and H5N1 was compared.

Results

Both H5N1 and H1N1 viruses remained infectious for periods of time as long as 19–25 days, respectively. However, within the same subtype, viruses grown on mammalian cells were more stable in water at 35°C than their counterparts grown on avian cells, even for viruses sharing the same genetic background.

Conclusions

This difference in virus stability outside the host is probably connected to the nature of the lipid bilayer taken from the cell or to the carbohydrate side chains of the virus surface glycoproteins. Moreover, the long-lasting survival time might have a critical role in the ecology of influenza viruses, especially for avian viruses.

Ancillary