Use of fractional factorial design to study the compatibility of viral ribonucleoprotein gene segments of human H7N9 virus and circulating human influenza subtypes
Version of Record online: 9 JUL 2014
© 2014 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
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Influenza and Other Respiratory Viruses
Volume 8, Issue 5, pages 580–584, September 2014
How to Cite
2014) Use of fractional factorial design to study the compatibility of viral ribonucleoprotein gene segments of human H7N9 virus and circulating human influenza subtypes. Influenza and Other Respiratory Viruses 8(5), 580–584.et al. (
- Issue online: 10 SEP 2014
- Version of Record online: 9 JUL 2014
- Manuscript Accepted: 13 JUN 2014
- Seed Funding Programme for Basic Research. Grant Number: HKU 201111159066
- National Institutes of Health. Grant Number: HHSN27220140006C
- Research Grant Council of Hong Kong. Grant Number: HKU 776611M
- Area of Excellence Scheme of the University. Grant Number: AoE/M-12/06
- Fractional factorial design;
- influenza polymerase
Avian H7N9 influenza viruses may pose a further threat to humans by reassortment with human viruses, which could lead to generation of novel reassortants with enhanced polymerase activity. We previously established a novel statistical approach to study the polymerase activity of reassorted vRNPs (Influenza Other Respir Viruses. 2013;7:969-78). Here, we report the use of this method to study recombinant vRNPs with subunits derived from human H1N1, H3N2, and H7N9 viruses. Our results demonstrate that some reassortant vRNPs with subunits derived from the H7N9 and other human viruses can have much higher polymerase activities than the wild-type levels.