Fluorescent in situ hybridization: an effective and less costly technique for genetic evaluation of products of conception in pregnancy losses

  1. Moshe D. Fejgin1,2,3,
  2. Meir Pomeranz1,
  3. Meytal Liberman2,
  4. Ami Fishman1,3,
  5. Aliza Amiel2,4,*

Article first published online: 11 AUG 2005

DOI: 10.1111/j.0001-6349.2005.00757.x

Issue

Acta Obstetricia et Gynecologica Scandinavica

Acta Obstetricia et Gynecologica Scandinavica

Volume 84, Issue 9, pages 860–863, September 2005

Additional Information

How to Cite

Fejgin, M. D., Pomeranz, M., Liberman, M., Fishman, A. and Amiel, A. (2005), Fluorescent in situ hybridization: an effective and less costly technique for genetic evaluation of products of conception in pregnancy losses. Acta Obstetricia et Gynecologica Scandinavica, 84: 860–863. doi: 10.1111/j.0001-6349.2005.00757.x

Author Information

  1. 1

    From the Department of Obstetrics and Gynecology and

  2. 2

    the Genetic Institute, Meir Hospital, Kfar-Saba, Sackler School of Medicine,

  3. 3

    Tel Aviv University, Tel Aviv, and

  4. 4

    Bar-Ilan University, Ramat-Gan, Israel

* *Aliza Amiel Genetic Institute Meir Hospital Kfar-Saba 44281 Israel e-mail: amielaliza@clalit.org.il

Publication History

  1. Issue published online: 11 AUG 2005
  2. Article first published online: 11 AUG 2005
  3. Submitted 23 June, 2004; Accepted 21 October, 2004

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Keywords:

  • placenta;
  • fluorescent in situ hybridization;
  • spontaneous abortion;
  • induced abortion;
  • aneuploidy

Objective.  In this study, we applied the fluorescent in situ hybridization (FISH) technique and compared the common numerical abnormalities with chromosomes 13, 16, 18, 21, X, and Y in spontaneous to artificial abortion. This would cover about 75% of the common aneuploidy in spontaneous abortion.

Methods.  Placentas were taken from 59 patients with a first trimester spontaneous abortion and 61 patients who underwent an elective first trimester pregnancy termination. The range of growth was from 5 to 12 gestational weeks. Placentas were processed according to direct chorionic villi preparation. Direct dual color FISH was performed according to Vysis protocol with the probes for the following chromosomes: 13, 16, 18, 21, X, and Y.

Results.  The aneuploidy rate in spontaneous abortion was 55.9% and in artificial abortion 8.2%. There was a significant difference between the two groups in the aneuploidy rate (P = 6 × 10−9).

Conclusion.  FISH is a rapid, efficient, and relatively inexpensive tool in detecting aneuploidy in placentas from cases of spontaneous abortions. Our rate of detected aneuploidy is compatible with other reports in which conventional cytogenetics was utilized.

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