Present address: Wolfson Brain Imaging Centre, Cambridge, CB2 2QQ, U.K.
High resolution NMR microscopy of plants and fungi
Article first published online: 26 APR 2004
Journal of Microscopy
Volume 214, Issue 2, pages 182–189, May 2004
How to Cite
Köckenberger, W., De Panfilis, C., Santoro, D., Dahiya, P. and Rawsthorne, S. (2004), High resolution NMR microscopy of plants and fungi. Journal of Microscopy, 214: 182–189. doi: 10.1111/j.0022-2720.2004.01351.x
- Issue published online: 26 APR 2004
- Article first published online: 26 APR 2004
- Received 20 January 2004; accepted 11 February 2004
- Brassica napus;
- metabolite mapping;
- NMR microscopy on plants;
- Penicillium aurantognisium;
- Zinnia elegans
Nuclear magnetic resonance (NMR) microscopy is a completely noninvasive technique that can be used to acquire images with high spatial resolution through opaque objects such as plant organs and tissue parts. The image contrast can be chosen to represent the anatomical details or to visualize the spatial distribution of a range of physico-chemical parameters such as the apparent diffusion constant of water or the velocity of water flow within plants in vivo. In addition, images can be generated which show the spatial distribution of metabolites. Furthermore, it is possible to detect chemical compounds labelled with the stable isotope 13C and to generate images showing the spatial distribution of the 13C label in the intact plant. The ability to monitor water flow and transport of 13C-labelled tracer in intact plants with NMR microscopy favours the use of this technique in the investigation of long-distance transport processes in plants. A short introduction into the technical principles of NMR microscopy is provided and the problems associated with applications to plants are summarized. The potential of the technique is explained with applications to Zinnia elegans plants, wheat grains and Brassica napus siliques.