SEPARATION OF CHLOROPHYLLS c1c2, AND c3 OF MARINE PHYTOPLANKTON BY REVERSED-PHASE-C18-HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY1

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  • 1

    Received 24 December 1991. Accepted 26 May 1992.

  • We are grateful to W. W. C. Gieskes, I. Jennes, S. Wright, and an anonymous referee for their helpful comments on the manuscript.

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ABSTRACT

We separated chlorophylls c1 c2, and c3 of marine phytoplankton together with other pigments by a modification of the commonly applied reversed-phase-C18-high-performance liquid chromatography (RP-C18-HPLC) method. However, the chlorophyll c-like pigment 2,4, Mg-divinylpheoporphyrin as monomethyl ester, co-eluted with chlorophyll c1. The method involves optimization of the mobile phase by using a very high ion strength solvent in combination with a high carbon loaded RP-C18 column. Fingerprints of the various taxonomic groups of algae can thus be developed in a single run, including separation of the carotenoids lutein and zeaxanthin.

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