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SEPARATION OF CHLOROPHYLLS c1c2, AND c3 OF MARINE PHYTOPLANKTON BY REVERSED-PHASE-C18-HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY†

  1. Gijsbert W. Kraay1,*,
  2. Manuel Zapata2,
  3. Marcel J. W. Veldhuis3

Article first published online: 26 OCT 2004

DOI: 10.1111/j.0022-3646.1992.00708.x

Issue

Journal of Phycology

Journal of Phycology

Volume 28, Issue 5, pages 708–712, October 1992

Additional Information

How to Cite

Kraay, G. W., Zapata, M. and Veldhuis, M. J. W. (1992), SEPARATION OF CHLOROPHYLLS c1c2, AND c3 OF MARINE PHYTOPLANKTON BY REVERSED-PHASE-C18-HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY. Journal of Phycology, 28: 708–712. doi: 10.1111/j.0022-3646.1992.00708.x

Author Information

  1. 1

    Netherlands Institute for Sea Research, P.O. Box 59, 1790 AB Den Burg, Texel, The Netherlands

  2. 2

    Centro Experimental de Vilaxoan Conselleria de Pesca, Xunta de Galicia, Apdo. 208, 36600 Vilagarcia de Arousa, Spain

  3. 3

    Netherlands Institute for Sea Research, P.O. Box 59, 1790 AB Den Burg, Texel, The Netherlands

* Address for reprint requests.

  • †

    Received 24 December 1991. Accepted 26 May 1992.

  • We are grateful to W. W. C. Gieskes, I. Jennes, S. Wright, and an anonymous referee for their helpful comments on the manuscript.

Publication History

  1. Issue published online: 26 OCT 2004
  2. Article first published online: 26 OCT 2004

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Keywords:

  • chlorophylls c1 c2, c3;
  • chromophyte phytoplankton;
  • pigment analysis;
  • RP-C18-HPLC

ABSTRACT

We separated chlorophylls c1 c2, and c3 of marine phytoplankton together with other pigments by a modification of the commonly applied reversed-phase-C18-high-performance liquid chromatography (RP-C18-HPLC) method. However, the chlorophyll c-like pigment 2,4, Mg-divinylpheoporphyrin as monomethyl ester, co-eluted with chlorophyll c1. The method involves optimization of the mobile phase by using a very high ion strength solvent in combination with a high carbon loaded RP-C18 column. Fingerprints of the various taxonomic groups of algae can thus be developed in a single run, including separation of the carotenoids lutein and zeaxanthin.

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