Received 24 December 1991. Accepted 26 May 1992.
SEPARATION OF CHLOROPHYLLS c1c2, AND c3 OF MARINE PHYTOPLANKTON BY REVERSED-PHASE-C18-HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY†
Article first published online: 26 OCT 2004
Journal of Phycology
Volume 28, Issue 5, pages 708–712, October 1992
How to Cite
Kraay, G. W., Zapata, M. and Veldhuis, M. J. W. (1992), SEPARATION OF CHLOROPHYLLS c1c2, AND c3 OF MARINE PHYTOPLANKTON BY REVERSED-PHASE-C18-HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY. Journal of Phycology, 28: 708–712. doi: 10.1111/j.0022-3646.1992.00708.x
We are grateful to W. W. C. Gieskes, I. Jennes, S. Wright, and an anonymous referee for their helpful comments on the manuscript.
- Issue published online: 26 OCT 2004
- Article first published online: 26 OCT 2004
- chlorophylls c1 c2, c3;
- chromophyte phytoplankton;
- pigment analysis;
We separated chlorophylls c1 c2, and c3 of marine phytoplankton together with other pigments by a modification of the commonly applied reversed-phase-C18-high-performance liquid chromatography (RP-C18-HPLC) method. However, the chlorophyll c-like pigment 2,4, Mg-divinylpheoporphyrin as monomethyl ester, co-eluted with chlorophyll c1. The method involves optimization of the mobile phase by using a very high ion strength solvent in combination with a high carbon loaded RP-C18 column. Fingerprints of the various taxonomic groups of algae can thus be developed in a single run, including separation of the carotenoids lutein and zeaxanthin.