Received 13 July 1992. Accepted 7 December 1992.
GEL PURIFICATION OF RED ALGAL GENOMIC DNA: AN INEXPENSIVE AND RAPID METHOD FOR THE ISOLATION OF POLYMERASE CHAIN REACTION-FRIENDLY DNA1
Article first published online: 20 OCT 2004
Journal of Phycology
Volume 29, Issue 2, pages 251–254, April 1993
How to Cite
Saunders, G. W. (1993), GEL PURIFICATION OF RED ALGAL GENOMIC DNA: AN INEXPENSIVE AND RAPID METHOD FOR THE ISOLATION OF POLYMERASE CHAIN REACTION-FRIENDLY DNA. Journal of Phycology, 29: 251–254. doi: 10.1111/j.0022-3646.1993.00251.x
I thank Carolyn Bird, Gerry Kraft, and Mohsin Patwary for reviewing this manuscript, two anonymous reviewers for many suggestions that greatly improved this manuscript, and M. Holloway and R. Waller for technical assistance. This research was supported by an Australian Research Council grant # AD9031748 to Drs. G. T. Kraft and A. F. Clarke and a Natural Sciences and Engineering Research Council of Canada postdoctoral fellowship to G.W.S.
- Issue published online: 20 OCT 2004
- Article first published online: 20 OCT 2004
- DNA isolation;
- polymerase chain reaction;
A simplified approach for the extraction of DNA from red algae in presented. Procedures are simple and fast, requiring a minimum of reagents and apparatus. The method involves an initial lysis step followed by an optional phenol/chloroform extraction. The final gel-purification step removes polymerase chain reaction-inhibiting polysaccharides from the DNA preparation. DNA is extracted as easily from dried algae as it is from snap-frozen, fresh material, thus greatly facilitating the collection and transport of algal samples.