GEL PURIFICATION OF RED ALGAL GENOMIC DNA: AN INEXPENSIVE AND RAPID METHOD FOR THE ISOLATION OF POLYMERASE CHAIN REACTION-FRIENDLY DNA1

Authors


  • 1

    Received 13 July 1992. Accepted 7 December 1992.

  • I thank Carolyn Bird, Gerry Kraft, and Mohsin Patwary for reviewing this manuscript, two anonymous reviewers for many suggestions that greatly improved this manuscript, and M. Holloway and R. Waller for technical assistance. This research was supported by an Australian Research Council grant # AD9031748 to Drs. G. T. Kraft and A. F. Clarke and a Natural Sciences and Engineering Research Council of Canada postdoctoral fellowship to G.W.S.

ABSTRACT

A simplified approach for the extraction of DNA from red algae in presented. Procedures are simple and fast, requiring a minimum of reagents and apparatus. The method involves an initial lysis step followed by an optional phenol/chloroform extraction. The final gel-purification step removes polymerase chain reaction-inhibiting polysaccharides from the DNA preparation. DNA is extracted as easily from dried algae as it is from snap-frozen, fresh material, thus greatly facilitating the collection and transport of algal samples.

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