Received 19 October 1992. Accepted 22 February 1993.
PRODUCTION OF AXENIC CULTURES OF MICROMONAS PUSILLA (PRASINOPHYCEAE) USING ANTIBIOTIC 1
Article first published online: 20 OCT 2004
Journal of Phycology
Volume 29, Issue 3, pages 385–387, June 1993
How to Cite
Cottrell, M. T. and Suttle, C. A. (1993), PRODUCTION OF AXENIC CULTURES OF MICROMONAS PUSILLA (PRASINOPHYCEAE) USING ANTIBIOTIC . Journal of Phycology, 29: 385–387. doi: 10.1111/j.0022-3646.1993.00385.x
- Issue published online: 20 OCT 2004
- Article first published online: 20 OCT 2004
- algal virus host;
- antibiotic resistance;
- axenic culture;
- Micromonas pusilla;
Bacteria-free cultures of the prasinophyte Micromonas pusilla (Butcher) Monton and Parke, UTEX LB 991, were produced by intially determining the effects of several antibiotics on the growth of this alga and then using a combination of these antibiotics to eliminate associated bacteria, Micrononas pusilla was resistant ot penicillin G, neomycin, gentamicin and streptomycin at bactericidal concentrations but sensitive to chloramphenicol and polymixin B. Passage of M. pusilla through the sequence of antibioties penicillin G → neomycin → gentamician → kanamycin resulted in an axenic culture of M, pusilla this method should be suitable for producing axenic culture of other strains of M. pusilla.