Received 15 September 1996. Accepted 15 February 1997.
NONDESTRUCTIVE TRACING OF MIGRATORY RHYTHMS OF INTERTIDAL BENTHIC MICROALGAE USING IN VIVO CHLOROPHYLL A FLUORESCENCE†‡
Article first published online: 28 JUN 2008
DOI: 10.1111/j.0022-3646.1997.00542.x
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How to Cite
Serôdio, J., Marques da Silva, J. and Catarino, F. (1997), NONDESTRUCTIVE TRACING OF MIGRATORY RHYTHMS OF INTERTIDAL BENTHIC MICROALGAE USING IN VIVO CHLOROPHYLL A FLUORESCENCE. Journal of Phycology, 33: 542–553. doi: 10.1111/j.0022-3646.1997.00542.x
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Abbreviations: a*(z), Optical absorption cross section normalized to Chl a of cells at depth z (m2· g Chl a−1); c(z). sediment Chl a content at depth z (g Chl a· m−3); Chl, chlorophyll; d, distance between the PAM fiberoptics and the sample surface (mm); E0 (d, z), scalar exciting spectral irradiance at depth z, at a distance d + z from the fiberoptics (W · m−2· nm−1); Ed(d, z), Ed, downwelling exciting spectral irradbance at depth z, at a distance d + z from the fiberoptics and at the fiberoptics level (W·m−2· nm−1); F(d), depth-integrated fluorescence intensity emitted by the ensemble of cells present in the sediment, which is detected by the fiberoptics at a distance d from the sediment surface (mV); F(d, z), fluorescence intensity emitted by cells present in a unit volume at depth z, which is detected at the fiberoptics level at a distance d + z from the sediment surface (mV); Fl (d, z), fluorescent radiance flux emitted by the cells present in a unit volume of sediment at depth z, at a distance d + z from the fiberoptics (W·m−3· nm−l); Fo, Fv, FM, dark-level (minimum), variable, and maximum values attainable by Chl a fluorescence intensity, here considered to be measured 1 mm from the sample surface (mV); Fu,(d, z), upwelling fluorescent spectral irradiance emitted at depth z, at a distance d + z from the fiberoptics (W·m−2·nm−1); φF(z), quantum yield of Chl a fluorescence at depth z (quanta emittedquanta absorbed−1); φFo, φFM, dark-level and maximum quantum yield of Chl a fluorescence (quanta emittedquanta absorbed−1); G, factor representing the conversion between detected fluorescent irradiance and fluorometer output (mV·W−1·m2·nm); kair, ksed, attenuation coefticients of exciting light and emitted fluorescence in the air and in the sediment (mm−1); LHCII, light-harvesting complex of phutosystem II; μ(z), average cosine (mean cosine of the average path direction of incident photons); nPSII(z), PSII concentration at depth z (PSII · m−3); PSII, photosystem II; Q(z), fractional reduction of emitted fluorescence due to intracellular reabsorption at depth z, rex, rem, fractional reflexion losses of exciting light and emitted fluorescence at the sediment-air (or sediment-water) interface; R(z), irradiance reflectance (ratio of upward to downward irradiance) at depth z; σPSII (z), functional absorption cross section of energy-absorbing pigments in LHCII at depth z (m2ΨII−1); z, depth below sediment surface (mm).
Publication History
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
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