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Downregulation of cell surface receptors by the K3 family of viral and cellular ubiquitin E3 ligases

Authors


* Paul J Lehner
Department of Medicine
Cambridge Institute for Medical Research
Addenbrooke's Hospital
Hills Road
Cambridge, CB2 2XY, UK
Tel.: +44 1223 762113
Fax: +44 1223 762640
E-mail: pjl30@cam.ac.uk

Abstract

Summary:  The mK3, K3, and K5 gene products from the γ2 group of γ-herpesviruses are the founding members of a family of membrane-associated ubiquitin E3 ligases. As part of the viral immunoevasion strategy, expression of these proteins results in a decrease in cell-surface major histocompatibility complex class I molecules and other immunoreceptors including intercellular adhesion molecule-1, CD86, and CD1d. These viral gene products all possess a characteristic cytosolic N-terminal RING-CH domain, responsible for ubiquitination of the target protein, and two membrane-spanning segments required for substrate specificity. For the majority of substrates, ubiquitination at the cell surface leads to rapid internalization and endolysosomal degradation, while mK3 ubiquitinates class I molecules associated with the peptide-loading complex resulting in proteasome-mediated degradation. Related viral genes with similar functions have been found in poxviruses, suggesting appropriation of these genes from the eukaryotic host. Ten membrane-associated RING-CH (MARCH) human genes with a similar organization have now been identified, and their overexpression leads to ubiquitination and downregulation of a variety of cell-surface immunoreceptors. While all the MARCH proteins are predicted to act as ubiquitin E3 ligases, their physiological role and substrates remain to be defined.

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