The use of genomewide ENU mutagenesis screens to unravel complex mammalian traits: identifying genes that regulate organ-specific and systemic autoimmunity
Article first published online: 28 MAR 2006
Volume 210, Issue 1, pages 27–39, April 2006
How to Cite
Hoyne, G. F. and Goodnow, C. C. (2006), The use of genomewide ENU mutagenesis screens to unravel complex mammalian traits: identifying genes that regulate organ-specific and systemic autoimmunity. Immunological Reviews, 210: 27–39. doi: 10.1111/j.0105-2896.2006.00363.x
- Issue published online: 28 MAR 2006
- Article first published online: 28 MAR 2006
Summary: T-cell development is perhaps one of the best understood processes of mammalian cell differentiation, as many of the genes and pathways have been identified. By contrast, relatively little is known about the genes and pathways involved in immunological tolerance to self-antigens. Here, we describe the challenges associated with a genomewide screen designed at identifying new immune regulatory genes that uses a model of organ-specific autoimmunity leading to type 1 diabetes. The successful propagation and identification of the new gene variants will shed light on the various developmental checkpoints in lymphocyte development that are crucial for establishing tolerance to self-antigens.