hTERT expression in melanocytic lesions: an immunohistochemical study on paraffin-embedded tissue
Article first published online: 16 NOV 2005
Journal of Cutaneous Pathology
Volume 32, Issue 10, pages 680–684, November 2005
How to Cite
Fullen, D. R., Zhu, W., Thomas, D. and Su, L. D. (2005), hTERT expression in melanocytic lesions: an immunohistochemical study on paraffin-embedded tissue. Journal of Cutaneous Pathology, 32: 680–684. doi: 10.1111/j.0303-6987.2005.00403.x
- Issue published online: 16 NOV 2005
- Article first published online: 16 NOV 2005
- Accepted for Publication March 14, 2005
Background: Telomerase plays a role in the immortalization of cells and carcinogenesis. Previous studies have yielded conflicting results on whether human telomerase RNA (hTER) expression differs in nevi, atypical nevi and melanomas using polymerase chain reaction-based telomeric repeat amplification protocol or in situ hybridization assays. The aim of this study was to evaluate human telomerase reverse transcriptase (hTERT) staining in melanocytic lesions on paraffin-embedded tissues.
Methods: Paraffin-embedded sections from 12 acquired nevi, seven dysplastic nevi, 11 Spitz nevi, eight primary invasive melanomas, and three metastatic melanomas were studied for staining intensity (0–3+) and percentage of labeled cells with anti-hTERT.
Results: hTERT staining was observed in most cells (>75%), in all but three lesions, and was of greater intensity in the nucleus, especially the nucleolus, compared with the cytoplasm. Spitz nevi tended to have weaker hTERT staining (mean = 1.7) compared with acquired nevi (mean = 2.2), dysplastic nevi (mean = 2.4), primary melanomas (mean = 2.4), or metastatic melanomas (mean = 3).
Conclusions: Although telomerase activity was weaker in Spitz nevi, there was overlap with other nevi and primary invasive melanomas in our small series. Thus, hTERT expression does not appear to be a reliable adjunct to the histological diagnosis of primary melanocytic lesions.