The data shown in this paper represent a part of a PhD thesis at the University of Regensburg, Germany.
Identification of differentially expressed genes in models of melanoma progression by cDNA array analysis: SPARC, MIF and a novel cathepsin protease characterize aggressive phenotypes
Article first published online: 10 DEC 2003
Volume 12, Issue 6, pages 761–771, December 2003
How to Cite
Rumpler, G., Becker, B., Hafner, C., McClelland, M., Stolz, W., Landthaler, M., Schmitt, R., Bosserhoff, A. and Vogt, T. (2003), Identification of differentially expressed genes in models of melanoma progression by cDNA array analysis: SPARC, MIF and a novel cathepsin protease characterize aggressive phenotypes. Experimental Dermatology, 12: 761–771. doi: 10.1111/j.0906-6705.2003.00082.x
- Issue published online: 10 DEC 2003
- Article first published online: 10 DEC 2003
- Accepted for publication 19 September 2002
- malignant melanoma;
- RNA-fingerprinting (RAP-PCR);
- gene expression
Abstract: Currently, the scale and consistency of changes of gene expression profiles in models of melanoma progression are largely unknown. Therefore, we investigated siblings of cell lines of malignant melanomas (MM), which have been selected by nude mouse passages for (a) increased tumorigenicity (local ECM-independent growth), (b) metastatic potential, or (c) selected for increased invasiveness using the Boyden chamber. cDNA array analysis surveying more than 27.000 transcripts per cell line showed that 1.5–2.8% of all detectable transcripts were consistently differentially regulated during the selection processes in those models. Using array analysis, we identified 33 individual transcripts that exhibited significant differential hybridization paralleling the increased aggressiveness of the selected progeny. Because some of those genes could play a significant functional role in the progression of MM, we additionally proved their regulative pattern using Northern blotting. Among others, progressive overexpression of osteonectin/SPARC, a molecule that is known to be involved in tissue remodeling and angiogenesis, was found in the selected offspring from all three experimental models and may therefore be considered as a potential marker for aggressive MM as well as a promising therapeutic target. We further show that the selection of MM cells for increased ECM-independent local growth was accompanied by overexpression of macrophage migration inhibiting factor (MIF), an important modulator of both cell cycle progression and angiogenesis, and cathepsin Z, a novel member of the family of matrix degrading proteinases.