Expression of vanilloid receptor subtype 1 in cutaneous sensory nerve fibers, mast cells, and epithelial cells of appendage structures

Authors

  • Sonja Ständer,

    Corresponding author
    1. Department of Dermatology, University of Münster, Münster, Germany;
    2. Ludwig Boltzmann Institute for Cell Biology and Immunobiology of the Skin, University of Münster, Münster, Germany;
      Sonja Ständer, MD
      Department of Dermatology
      University Hospital of Münster
      Von-Esmarchstr. 58
      D-48149 Münster
      Germany
      Tel.: +49 251 835 6504
      Fax: +49 251 835 6522
      e-mail: sonja.staender@uni-muenster.de
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    • *

      These authors contributed equally to the work.

  • Corinna Moormann,

    1. Ludwig Boltzmann Institute for Cell Biology and Immunobiology of the Skin, University of Münster, Münster, Germany;
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    • *

      These authors contributed equally to the work.

  • Mark Schumacher,

    1. Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA, USA;
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  • Jörg Buddenkotte,

    1. Ludwig Boltzmann Institute for Cell Biology and Immunobiology of the Skin, University of Münster, Münster, Germany;
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  • Metin Artuc,

    1. Department of Dermatology, Charite, Humboldt-University of Berlin, Berlin, Germany;
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  • Victoria Shpacovitch,

    1. Ludwig Boltzmann Institute for Cell Biology and Immunobiology of the Skin, University of Münster, Münster, Germany;
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  • Thomas Brzoska,

    1. Ludwig Boltzmann Institute for Cell Biology and Immunobiology of the Skin, University of Münster, Münster, Germany;
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  • Undine Lippert,

    1. Max-Planck-Institute for Biophysical Chemistry, University of Göttingen, Göttingen, Germany
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  • Beate M. Henz,

    1. Department of Dermatology, Charite, Humboldt-University of Berlin, Berlin, Germany;
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  • Thomas A. Luger,

    1. Department of Dermatology, University of Münster, Münster, Germany;
    2. Ludwig Boltzmann Institute for Cell Biology and Immunobiology of the Skin, University of Münster, Münster, Germany;
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  • Dieter Metze,

    1. Department of Dermatology, University of Münster, Münster, Germany;
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  • Martin Steinhoff

    1. Department of Dermatology, University of Münster, Münster, Germany;
    2. Ludwig Boltzmann Institute for Cell Biology and Immunobiology of the Skin, University of Münster, Münster, Germany;
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Sonja Ständer, MD
Department of Dermatology
University Hospital of Münster
Von-Esmarchstr. 58
D-48149 Münster
Germany
Tel.: +49 251 835 6504
Fax: +49 251 835 6522
e-mail: sonja.staender@uni-muenster.de

Abstract

Abstract:  The vanilloid receptor subtype 1 (VR1)/(TRPV1), binding capsaicin, is a non-selective cation channel that recently has been shown in human keratinocytes in vitro and in vivo. However, a description of VR1 localization in other cutaneous compartments in particular cutaneous nerve fibers is still lacking. We therefore investigated VR1 immunoreactivity as well as mRNA and protein expression in a series (n = 26) of normal (n = 7), diseased (n = 13) [prurigo nodularis (PN) (n = 10), generalized pruritus (n = 1), and mastocytosis (n = 2)], and capsaicin-treated human skin (n = 6). VR1 immunoreactivity could be observed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands. Upon reverse transcriptase-polymerase chain reaction and Western blot analysis, VR1 was detected in mast cells and keratinocytes from human skin. In pruritic skin of PN, VR1 expression was highly increased in epidermal keratinocytes and nerve fibers, which was normalized after capsaicin application. During capsaicin therapy, a reduction of neuropeptides (substance P, calcitonin gene-related peptide) was observed. After cessation of capsaicin therapy, neuropeptides re-accumulated in skin nerves. In conclusion, VR1 is widely distributed in the skin, suggesting a major role for this receptor, e.g. in nociception and neurogenic inflammation.

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