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Abstract

  1. Top of page
  2. Abstract
  3. Amphibians and Hanski's four metapopulation conditions
  4. Amphibian site loyalty and movement
  5. Case study
  6. Conclusions
  7. Acknowledgements
  8. References
  9. Appendix

Amphibians are frequently characterized as having limited dispersal abilities, strong site fidelity and spatially disjunct breeding habitat. As such, pond-breeding species are often alleged to form metapopulations. Amphibian species worldwide appear to be suffering population level declines caused, at least in part, by the degradation and fragmentation of habitat and the intervening areas between habitat patches. If the simplification of amphibians occupying metapopulations is accurate, then a regionally based conservation strategy, informed by metapopulation theory, is a powerful tool to estimate the isolation and extinction risk of ponds or populations. However, to date no attempt to assess the class-wide generalization of amphibian populations as metapopulations has been made. We reviewed the literature on amphibians as metapopulations (53 journal articles or theses) and amphibian dispersal (166 journal articles or theses for 53 anuran species and 37 salamander species) to evaluate whether the conditions for metapopulation structure had been tested, whether pond isolation was based only on the assumption of limited dispersal, and whether amphibian dispersal was uniformly limited. We found that in the majority of cases (74%) the assumptions of the metapopulation paradigm were not tested. Breeding patch isolation via limited dispersal and/or strong site fidelity was the most frequently implicated or tested metapopulation condition, however we found strong evidence that amphibian dispersal is not as uniformly limited as is often thought. The frequency distribution of maximum movements for anurans and salamanders was well described by an inverse power law. This relationship predicts that distances beneath 11–13 and 8–9 km, respectively, are in a range that they may receive one emigrating individual. Populations isolated by distances approaching this range are perhaps more likely to exhibit metapopulation structure than less isolated populations. Those studies that covered larger areas also tended to report longer maximum movement distances – a pattern with implications for the design of mark-recapture studies. Caution should be exercised in the application of the metapopulation approach to amphibian population conservation. Some amphibian populations are structured as metapopulations – but not all.

The importance of the spatial element in ecology has long been recognized (Andrewartha and Birch 1954), but its consideration has undergone a period of distinct growth in the past thirty years (Hanski 1999) with increased emphasis paid to concepts such as the spatial nature of population dynamics and the spatial partitioning of populations’ genetic variability. The metapopulation approach, first outlined by Levins (1969, 1970), has been especially insightful for the development of spatial ecology and its application to conservation. In simple terms, a metapopulation is a collection of partially isolated breeding habitat patches, connected by occasionally dispersing individuals whereby each patch exists with a substantial extinction probability. Thus, long-term persistence occurs only at the regional level of the metapopulation. There are currently many variants of the metapopulation concept ranging from very simple models utilizing a minimum of data (Levins 1969) to much more complex models which incorporate many environmental variables (Sjögren Gulve and Ray 1996, Harrison and Taylor 1997, Hanski 1999). The most useful function of this continuum of metapopulation theory has been to integrate spatially structured interactions between local populations with processes occurring within populations and thereby enable better assessment of population viability.

Models more realistic than the initial Levins approach consider the effects of patch area, shape and isolation, and the effect of the non-habitat between patches on the likelihood of patch extinction or colonization (Hanski 1999). This addition of realism has been accompanied by an exponential increase in the number of biological systems examined for metapopulation structure (Hanski 1999). However, rapidly increasing empirical use of metapopulation concepts, without clear tests of the theory's applicability, may reduce the precision with which the term metapopulation is used (Freckleton and Watkinson 2003, Pannell and Obbard 2003). For instance, conservation strategies may be misdirected if similar species are considered a priori to exhibit similar population structure and dynamics in the absence of evidence to the contrary or if departures from simple metapopulation models are not clearly detailed (Hanski and Simberloff 1997). It has been stated that because metapopulation dynamics are concerned mostly with the presence or absence of species in local populations, the sampling of any specific local populations would not need to be as intense (Alford and Richards 1999). Clearly this will be a decision with dire consequences if there is no true metapopulation structure at all. Such a conclusion is only valid for a simple metapopulation and therefore it would also be a mistake if a more extended, complex metapopulation was actually modeled (Harrison and Taylor 1997). Therefore, the issue of whether or not populations of amphibians, or any taxa, form a metapopulation is not merely semantic but is relevant to their conservation and management.

Certainly, where disjunct breeding patches contain individual populations that exist in a shifting balance between extinctions and recolonisations via dispersing individuals the metapopulation approach is an attractive theoretical construct (Hanski 1999). Many temperate amphibians do use spatially disjunct breeding habitat (Duellman and Trueb 1986), and are often regarded as poor-dispersers with high site fidelity (Duellman and Trueb 1986, Sinsch 1990, Blaustein et al. 1994, Beebee 1996, Berry 2001). In light of these observations, it appears highly probable that amphibian populations likely do operate as metapopulations (Harrison 1991, Alford and Richards 1999, Marsh and Trenham 2001). “…Our review of the literature suggests that many if not most amphibians exist in metapopulations” (Alford and Richards 1999). Yet the assumptions of limited dispersal, high site fidelity and evident metapopulation structure in amphibians have not been stringently tested, and we do so here.

We examined the literature for all references to amphibians as metapopulations using Current Contents, Scientific Citation Index, and other published reference lists. We tested whether the published literature for amphibians had addressed the four conditions deemed necessary for the existence of simple metapopulation structure (Hanski et al. 1995, Hanski 1999), and whether amphibians are indeed of low vagility and high site loyalty. Although the metapopulation concept has been extended to include population structures not described by these conditions (Harrison and Taylor 1997, Hanski 1999), our analysis is restricted to these simple conditions for the single reason that simple, stochastic metapopulations are likely good models for real populations living in highly fragmented landscapes (Ovaskainen and Hanski 2004) – a situation which includes many temperate, pond-breeding amphibians (Marsh and Trenham 2001). If populations inhabiting habitat patches are best described by simple metapopulation models that are described by Hanski's four conditions, then the collection of empirical data, advantageous to conservation and management, is relatively simple. Thus, to review the applicability of the 4 conditions of a simple metapopulation to amphibian ecology is timely and necessary.

Our secondary focus is upon Hanski's 3rd condition of limited dispersal ability. Previous reviews of amphibians and the metapopulation concept (Alford and Richards 1999, Marsh and Trenham 2001) focused primarily on the Hanski's 1st condition – that breeding ponds constitute separate and individual breeding populations. While both reviews identified the importance of dispersal for determining the range of recolonisation – a comprehensive review of dispersal abilities in relation to the metapopulation concept was not completed and our review is therefore complimentary. It is also timely, for most metapopulation models assume that metapopulation dynamics are dominated by short distance movements (Hanski 1999, Baguette 2003), and if we underestimate the likelihood of long distance dispersal we risk either incorrectly estimating the scale of a metapopulation effect, or incorrectly attributing metapopulation structure to a system where local dynamics are more important than regional (i.e. not a metapopulation). Thus, correctly estimating long-distance dispersal lies at the heart of determining the appropriate scale of a metapopulation approach.

We compiled a comprehensive list of the longest distances moved by amphibians in both mark-recapture and displacement studies (Tables 3 and 4). To test whether the low-vagility hypothesis was caused by a lack of long-distance dispersal data in amphibians caused by small study areas we subsequently compared these movement distances to the maximum distance covered by the field site. If not recorded directly by the author, the maximum observable distance was most frequently entered as the diagonal of the figure documenting the study site – likely a liberal interpretation of maximum distance measurable.

Table 3.  Maximum dispersal distance recorded, longest distance able to be measured in the reported study site, methodology used for distance estimate and sex and age of dispersing individual for 37 salamander species from 64 reports. Methodological abbreviations for capture techniques are as follows (Mark-recapture (MRR), toeclip (TOE), elastomer implant (ELAS), radio telemetry (RAD), passive integrated transponder (PIT), Radioactively tagged with Cobalt 60 (Co60), radioactively tagged with Tantalum 182 (Ta182), freeze branded (BRAND), distance to nearest wetland (DIST to WETLAND), microtagged (MICROTAG), individually specific skin pattern (PATTERN), displacement (DISP), tattoo (TAT), yearly rate of movement from introduction (RATE), jaw tag (JAW), spool of thread attached to animal and followed (SPOOL). When the largest reported dispersal distance is larger than the reported, or measured, longest axis of the study the ratio of study site long axis: maximum distance may exceed one. Such a situation might occur when an animal was recaptured during an occasional survey outside the study area as in Griffiths (1984), and Dole (1971). Thus values greater than one can be understood to support our hypothesis that animals are currently being located at the maximum distances being searched.
SpeciesMax distance recorded (m)Longest measure of study site (m)Ratio study site to max distanceMethodAgeReference
Ambystoma californiense670.003000.000.22MRR(PIT/TOE)AdultTrenham et al. 2001
Ambystoma californiense129.00  UNMARKAdultLoredo et al. 1997
Ambystoma jeffersonianum250.00567.000.44MRR(Co60)AdultDouglas and Monroe 1981
Ambystoma jeffersonianum1610.00  UNKNOWNUnknownBishops 1941
Ambystoma jeffersonianum625.00  MRR(Ta182)AdultWilliams 1973
Ambystoma jeffersonianum231.00  UNMARKUnknownWacasey 1961
Ambystoma laterale405.00692.000.59RADAdultFaccio 2003
Ambystoma macrodactylum1170.0039000.000.03UNMARKAdultFunk and Dunlap 1999
Ambystoma maculatum220.00567.000.39MRR(Co60)AdultDouglas and Monroe 1981
Ambystoma maculatum125.00  MRR(Ta182)AdultWilliams 1973
Ambystoma maculatum500.00500.001.00MRR(TOE)Adult femaleShoop 1968
Ambystoma maculatum402.00  UNMARKAdultGordon 1968
Ambystoma maculatum756.00711.001.06RADAdult maleMadison 1997
Ambystoma maculatum200.00  UNMARKUnknownWacasey 1961
Ambystoma maculatum249.00  MRR(Co60)UnknownKleeberger and Werner 1983
Ambystoma opacum30.00567.000.05MRR(Co60)AdultDouglas and Monroe 1981
Ambystoma opacum450.00  MRR(Ta182)AdultWilliams 1973
Ambystoma opacum1000.00600.001.67MRRJuvenilePechmann et al. 2001
Ambystoma talpoideum280.00400.000.7MRR(Ta182)Adult maleSemlitsch 1981
Ambystoma talpoideum1000.00600.001.67MRRJuvenilePechmann et al. 2001
Ambystoma texanum125.00  MRR(Ta182)AdultWilliams 1973
Ambystoma tigrinum600.00600.001.00MRRUnknownPechmann et al. 2001
Ambystoma tigrinum12.40  MRR(TOE)AdultSemlitsch 1983
Ambystoma tigrinum485.20  MRR(RAD)Adult maleMadison and Farrand 1997
Amphiuma tridactylum297.00  MRR(TAG)AdultCagle 1948
Aneides aeneus106.00  MRR(TOE)Adult femaleGordon 1961
Aneides aeneus15.24  UNMARKAdultWilliams and Gordon 1961
Aneides vagrans20.301300.000.02MRR(TOE)UnknownDavis 2002
Cryptobranchus alleganiensis990.00  MRR(TAG)AdultNickerson and Mays 1973
Cryptobranchus alleganiensis85.00100.000.85MRR(BRAND)Adult malePeterson 1987
Cryptobranchus alleganiensis900.00  UNKNOWNUnknownWiggs 1977
Desmognathus fuscus19.8050.000.40MRR(Co60)AdultAshton 1975
Desmognathus fuscus17.20  MRR(Co60)AdultBarbour et al. 1969
Desmognathus fuscus40.00  MRR(TOE)AdultBarthalamus and Bellis 1972
Desmognathus quadromaculatus25.0040.001.00MRR(ELAST)LarvaeFreeman 2003
Dicamptodon tenebrosus66.25180.000.14RADAdultJohnston and Frid 2002
Dicamptodon tenebrosus63.00120.000.53MRR(TOE/PIT)LarvaeFerguson 2000
Ensatina eschscholtzii platensis150.40  MARK(SPOT)Adult maleStaub et al. 1995
Eurycea bislineata420.00  UNMARKLarvaeJohnson and Goldberg 1975
Eurycea quadridigitata600.00600.001.00MRRJuvenilePechmann et al. 2001
Gyrinophilus porphyriticus490.001000.000.49MRR(ELAS)UnknownLowe 2003
Hynobius nebulosus tokyoensis90.00332.000.27MRR(TOE)AdultKusano and Miyashita 1984
Necturus beyeri65.00  MRR(TOE)JuvenileShoop and Gunning 1967
Necturus maculosus256.00  MRR(TOE)Adult femaleShoop and Gunning 1967
Notophthalmus perstriatus709.00  DIST to WETLANDUnknownDodd 1996
Notophthalmus viridescens1000.006522.000.15MRR(SPOT)Adult femaleGill 1978
Plethodon cinerus90.0090.001.00MRR(Co60)AdultKleeberger and Werner 1982
Plethodon glutinosus91.5032.002.86MRR(TOE)Adult male and femaleWells and Wells 1976
Plethodon jordani60.00  MRR(Ta182)Adult femaleMadison and Shoop 1970
Plethodon jordani300.00300.001.00MRR(TOE/DISP)AdultMadison 1969
Plethodon kentucki13.9515.000.93MRR(PATTERN)Adult femaleMarvin 1998
Plethodon vehiculum8.5014.000.61MRR(TOE)Adult maleOvaska 1988
Rhyacotritdon cascade6.0911.660.52MRR(PATTERN)Adult femaleNijhuia and Kaplan 1998
Salamandra atra aurorae30.00933.000.03MRR(PATTERN)AdultBonato and Fracasso 2003
Salamandra salamandra30.00  MRR(PATTERN)Adult males and femaleRebelo and Leclair 2003
Taricha rivularis12874.7512874.001.00MRR(AMP)AdultTwitty et al. 1967
Taricha rivularis4023.364023.361.00MRR(AMP)AdultTwitty et al. 1964
Triturus alpestris500.00  MRR(PIT/TAT)AdultPerret et al. 2003
Triturus alpestris200.00  MRR(TOE)Adult male and femaleJoly and Grolet 1996
Triturus carnifex299.00637.000.47MRR(RAD)Adult male and femaleSchabetsberger et al. 2004
Triturus vulgaris80.0030.902.59MRR(TOE)Adult femaleGriffiths 1984
Triturus vulgaris182.00400.000.46UNMARKUnknownWarwick 1949
Triturus vulgaris50.00  MRRJuvenileBell 1977
Triturus vulgaris123.00  MRR(TOE)Adult femaleDolmen 1981
Table 4.  Maximum dispersal distance recorded, longest distance able to be measured in the reported study site, methodology used for distance estimate and sex and age of dispersing individual for 53 anuran species from 102 reports. Abbreviations for capture methodology are as in Table 3.
SpeciesMax distance recorded (m)Longest measure of study site (m)Ratio study site to max distanceMethodAgeReference
Ascaphus truei360.002800.000.13MRR(TOE)JuvenileDaugherty and Sheldon 1982
Atelopus oxyrhynchus55.00  MRR(TOE)Adult maleDole and Durant 1974
Atelopus varius20.0020.001.00MRR(TOE)Adult maleCrump 1986
Bombina variegata312.801000.000.31MRR(TOE)Adult maleBeshkov and Jameson 1980
Bufo americanus6437.38  UNMARKEDNot reportedHamilton 1934
Bufo americanus235.00235.001.00MRR(TOE/DISP)AdultDole 1972
Bufo americanus1000.00  RATE/INTROUnknownMaunder 1983
Bufo americanus548.641254.030.44MRR(TOE)Adult maleBlair 1943
Bufo americanus594.00  MRR(TOE/DISP)AdultOldham 1966
Bufo americanus4023.36  UNMARKEDNot reportedMaynard 1934
Bufo baxteri99.97  MRR(TOE)Adult maleCarpenter 1954
Bufo baxteri423.80  MRR(RAD)Adult maleParker and Anderson 2003
Bufo boreas2440.00  MRR(RAD)Adult FemaleBartlet 2000
Bufo boreas6000.0015000.000.40UNREPORTEDUnreportedMuths et al. 2003
Bufo boreas2324.20  MRR(RAD)Adult femaleMuths 2003
Bufo boreas200.00200.001.00MRR(TOE/DISP)AdultTracy and Dole 1969
Bufo bufo3000.00  MRR(DISP)UnknownHeusser 1969
Bufo bufo3621.02  MRR(TOE)AdultMoore 1954
Bufo bufo985.008485.28 MRR(RAD)Adult femaleSinsch 1988
Bufo bufo118.00  MRR(TOE)Not reportedParker and Gittins 1979
Bufo bufo500.00  MRR(TOE)Not reportedHaapanen 1974
Bufo bufo1760.00  MRRAdultSinsch 1989
Bufo calamita4411.003605.55 MRR(RAD)Adult femaleMiaud et al. 2000
Bufo calamita400.002828.43 MRR(MICROTAG)JuvenileSinsch 1997
Bufo calamita2600.00  MRR(RAD)AdultSinsch 1992
Bufo fowleri1650.002000.00 MRR(TOE)JuvenileBreden 1987
Bufo fowleri312.001770.00 MRR(TOE)AdultClarke 1974
Bufo fowleri335.281254.03 MRR(TOE)Adult maleBlair 1943
Bufo fowleri1600.00  MRR(TAG)AdultStille 1952
Bufo fowleri1280.16  UNKNOWNUnreportedNichols 1937
Bufo fowleri34000.0034000.00 MRR(TOE)Adult FemaleSmith and Green (unpubl.)
Bufo fowleri100.00  MRR(TOE)AdultFerguson 1960
Bufo hemiophrys342.00  MRR(TOE/Ta182)AdultBreckenridge and Tester 1961
Bufo japonicus formosus260.00500.00 MRR(RAD)AdultKusano et al. 1995
Bufo marinus15100.00  RATEUnknownEasteal and Floyd 1986
Bufo marinus i35000.00  RATEUnknownFreeland and Martin 1985
Bufo marinus1300.00  MRR(RAD)AdultSchwarzkopf and Alford 2002
Bufo punctatus365.761105.300.33MRR(TOE)AdultTurner 1959
Bufo punctatus822.96  MRR(TOE)Adult femaleTevis 1966
Bufo punctatus900.00900.001.00MRR(TOE/DISP)AdultWeintraub 1974
Bufo terrestris1609.342449.290.66MRR(TAG)AdultBogert 1947
Bufo valliceps800.00  MRR(TOE)Adult maleBlair 1943
Dendrobates pumilio20.0020.001.00MRR(TOE/DISP)Adult femaleMcVey et al. 1981
Eleutherodactylus coqui6.68  MRR(ELASTOMER)AdultsWoolbright 1985
Eleutherodactylus fitzingeri35.00  MRR(PATTERN)UnreportedHobel 1999
Gastrophryne olivacea609.60  UNKNOWNUnknownFitch 1956
Gastrophryne carolinensis914.00  DIST to WETLANDUnknownDodd 1996
Geocrina alba39.00150.000.26MRR(TOE)Adult maleDriscoll 1997
Geocrina vitellina49.00150.000.33MRR(TOE)Adult maleDriscoll 1997
Heleioporus australiacus463.00  MRR(RAD)Adult maleLemckert and Brassil 2003
Heleioporus eyrei2500.00  DIST to WETLANDUnknownBamford 1992
Hyla arborea12600.00  UNREPORTEDUnreportedStumpel and Hanekamp 1986
Hyla arborea12570.0018000.000.70MRRUnreportedVos et al. 2000
Hyla arborea1500.00  UNREPORTEDUnreportedCarlson and Edenhamn 2000
Hyla arborea3750.00  UNKNOWNUnknownClausnitzer and Clausnitzer 1984
Hyla regilla1000.00 1.00MRR(TOE/DISP)Adult maleJameson 1957
Hyla regilla1900.00  RATE/INTROUnreportedReimchen 1990
Hyla versicolor125.00 0.96MRR(TOE)JuvenileRoble 1979
Leiopelma hochstetteri12.65 0.11MRR(TOE)AdultTessier et al. 1991
Mixophyes iteratus2000.00 0.05MRR(RAD)Adult femaleLemckert and Brassil 2000
Pelobates fuscus500.00  MRR(PIT)Adult male and femaleHels 2002
Phyllomedusa bicolor46.60  MRR(SPOOL)Adult maleNeckel de Oliveira 1996
Physaleemus pustulosus820.00 0.27MRR(TOE)AdultMarsh et al. 1999
Pseudacris triseriata213.00 1.58MRR(Co60)AdultKramer 1973
Pseudacris maculata685.80  UNKNOWNUnknownSpencer 1964
Rana arvalis7600.00  RATE (GENETIC)Nuclear markerVos et al. 2001
Rana aurora300.00 0.38MRR(TOE)Adult maleCalef 1973
Rana aurora aurora24000.00  MRR(PIT)Adult femaleHayes et al. 2001
Rana aurora aurora914.40  UNMARKEDAdultDumas 1966
Rana aurora draytonni3600.00 0.72MRR(RAD)AdultBulger et al. 2003
Rana berlandieri16000.00  RATEAdult malePlatx et al. 1990
Rana catesbeiana i1600.20  MRR(JAW)AdultIngram and Raney 1943
Rana catesbeiana914.401363.760.67MRR(JAW)UnreprotedRaney 1940
Rana catesbeiana966.00  MRR(JAW)AdultWillis et al. 1956
Rana clamitans600.0  MRR(TOE)Adult femaleMartof 1953
Rana clamitans560.00  MRR(RAD)AdultLamoureux and Madison 1999
Rana clamitans4800.00  MRR(TOE)JuvenileSchroeder 1976
Rana dalmatina300.00  UNMARKAdultPonsero and Joly 1998
Rana lessonae1200.001000.001.20MRR(TOE)JuvenileSjögren Gulve 1988
Rana lessonae1760.002830.000.62MRR(PIT)AdultHolenweg Peter 2001
Rana lessonae15000.00  MRRUnknownTunner 1992
Rana lutieventris6500.00  MRRAdultEngle 2001
Rana lutieventrisi2066.003905.000.53MRR(TOE/RAD)AdultfemalePilliod et al. 2002
Rana lutieventris560.00  MRR(RAD)Adult femaleBull and Hayes 2001
Rana mucosa1020.001414.210.72MRR(PIT)Adult femalePope and Matthews 2001
Rana pipiens3218.69  MRR(TOE)AdultMerrell 1970
Rana pipiens160.007.20.000.22MRR(TOE)Adult femaleDole 1965
Rana pipiens1744.00  MRR(TOE)AdultDole 1968
Rana pipiens8000.008000.001.00MRR(TOE)JuvenileSeburn et al. 1997
Rana pipiens5200.00720.007.22MRR(TOE)Juvenile maleDole 1971
Rana pretiosa1280.162133.600.60MRR(TOE)AdultTurner 1960
Rana pretiosa45.11  MRR(TOE)Adult maleCarpenter 1954
Rana ridibunda1760.002830.000.62MRR(PIT)AdultHolenweg Peter 2001
Rana sevosa299.001202.000.25MRR(RAD)AdultRichter et al. 2001
Rana sevosa2000.00  MRRAdult femaleFranz et al. 1988
Rana sevosa1600.00  UNMARKEDNot reportedCarr 1940
Rana sylvatica60.00186.000.32UNMARKEDNot reportedRegosin et al. 2003
Rana sylvatica500.00  MRR(TOE)AdultHoward and Kluge 1985
Rana sylvatica2530.002287.001.11MRR(TOE)JuvenileBerven and Grudzien 1990
Rana sylvatica89.6165.841.36MRR(TOE)AdultBellis 1965
Rana temporaria460.00  MRR(RAD)AdultSeitz et al. 1992
Syrrhophus marnocki380.39  MRR(TOE)AdultJameson 1955
Scaphious holbrooki825.00  MRR(TOE)AdultPearson 1955

The title of our review was inspired by that of Freckleton and Watkinson (2003) who have asked a similar question of the utility of the metapopulation concept to plant ecology.

Amphibians and Hanski's four metapopulation conditions

  1. Top of page
  2. Abstract
  3. Amphibians and Hanski's four metapopulation conditions
  4. Amphibian site loyalty and movement
  5. Case study
  6. Conclusions
  7. Acknowledgements
  8. References
  9. Appendix

Hanski outlines four conditions necessary to demonstrate the existence of a metapopulation effect (Hanski and Kuussaari 1995, Hanski et al. 1995, Hanski 1999): 1) habitat patches support local breeding populations, 2) no single population is large enough to ensure long-term survival, 3) patches are not too isolated to prevent recolonisation, and 4) local dynamics are sufficiently asynchronous to make simultaneous extinction of all local populations unlikely. Someone who observes a species with high site-fidelity, limited dispersal and apparently disjunct breeding patches, may frequently and implicitly, evaluate (or accept) these assumptions a priori. Through an examination of studies in the literature involving amphibians where the term metapopulation or population subdivision was used, we examined whether these studies had tested, not tested or assumed the importance of each of these conditions (Appendix 1). In some cases, it was unclear whether a study had “not tested” a Hanski Condition or whether it had been “assumed” to be accurate. Examples of two common differences occurred where a study involved a genetic analysis of metapopulation structure, (Rowe et al. 2000), or where a study was dependant on the assumption of limited dispersal. In the first scenario of a genetic metapopulation Condition 1 (that habitat patches support local breeding populations) is mathematically necessary to support the existence of a metapopulation, and was therefore considered as assumed, rather than not tested. In the second scenario of limited dispersal, Condition 3 (limited dispersal with the potential for recolonisation), was coded as assumed if there was an implication in the manuscript text that dispersal beyond a certain distance was expected to be impossible (Conroy and Brook 2003, Woodford and Meyer 2003).

Clearly there are a wide range of population conditions that have been called, “metapopulations” (Harrison and Taylor 1997), and we never suggest that the simple, stochastic type of metapopulation described by Hanski's conditions is the only type of metapopulation that exists. Rather, our goal was to investigate the frequency with which these simple models were actually tested by using a diverse taxa for which it has been suggested that the simple metapopulation was likely the predominant population condition (Harrison 1991, Alford and Richards 1999). Additionally, it was our hope to focus attention on the increasingly extended definition of the term metapopulation. We agree with Pannell and Obbard (2003) that the term, metapopulation should not be extended to include every instance where a species inhabits discrete habitat patches that may or may not be involved in population structure. If the term is extended until it includes practically all situations then it's utility is compromised as it is no longer falsifiable.

In our review we examined all instances where metapopulation was used in the title, abstract or keywords of journal articles or theses that studied amphibians. We included those articles that did not explicitly test a Hanski style metapopulation – because this manner of untested, a priori, defining of metapopulations is part of the trend we examine, and wish to draw attention to. In the instance where authors relied on previously published material we considered that condition to have been tested.

The majority (73.6%) of the possible 212 Hanski metapopulation conditions (53 articles×four conditions) were assumed or untested in the amphibian as metapopulation literature (Table 1). The most frequently tested condition was of isolation, although the dispersal ability of the amphibian species was rarely tested directly. Even though dispersal was indirectly estimated with genetics in 45% of the cases, the a priori assumption was that the amphibian was of low vagility. Any distance larger than one kilometre (Berven and Grudzien 1990, Sjögren 1991, Waldick 1997, Vos and Chardon 1998, Newman and Squire 2001, Conroy and Brook 2003), two kilometres (Hranitz and Diehl 2000), or several hundred meters (Reading et al. 1991, Skelly, et al. 1999) were referred to as critical distances beyond which amphibian dispersal would not penetrate. In fact, of the 53-amphibian/metapopulation studies, all explicitly tested or assumed that ponds were isolated due to the limited dispersal and/or high site fidelity of amphibians. 42 of 53 studies (79%) implicated this limited dispersal as the, or one of the, primary rationales behind the utility of the metapopulation process. Interestingly, studies that rejected the metapopulation paradigm (10/53) did so because there was judged to be too much dispersal among patches.

Table 1.  Literature review of the metapopulation paradigm in amphibian ecology. Fifty-three studies regarding amphibians and population sub-division were examined regarding their explicit or implicit testing of the four conditions necessary for a metapopulation effect (Hanski et al. 1995).
 Habitat patches support local breeding populationNo single population is large enough to ensure long-term survivalPatches are not too isolated to prevent recolonisation: isolation due to limited dispersalLocal dynamics are sufficiently asynchronous to make simultaneous extinction of all local population unlikely
Not tested2037045
Tested1110323
Assumed226215

Amphibian site loyalty and movement

  1. Top of page
  2. Abstract
  3. Amphibians and Hanski's four metapopulation conditions
  4. Amphibian site loyalty and movement
  5. Case study
  6. Conclusions
  7. Acknowledgements
  8. References
  9. Appendix

The literature contains many references that explain the poor dispersal ability of amphibians as being a consequence of their physiology and behavior (Duellman and Trueb 1986, Sinsch 1990, Blaustein et al. 1994). Amphibian skin is highly permeable and amphibians therefore have a stringent dependence on moisture (Duellman and Trueb 1986). Additionally, amphibians can show extreme site loyalty (Blaustein et al. 1994). Many individual studies have demonstrated that amphibians are found at the same location between census years and, where individuals have been followed through time, their movement is limited (Sinsch 1990) (Table 2).

Table 2.  Examples of site fidelity in amphibians.
SpeciesFidelitySource
Triturus vulgarisNot a single one of 2500 marked individuals changed breeding ponds between seasons.Bell 1977
Plethodon cinerusPhilopatric to the same cover object between years.Placyk and Graves 2001
Triturus vulgarisPhilopatric to the same cover object between years.Dolmen 1981
Bufo bufo93% of females and 96% of males that survived between years, returned to the same breeding ponds.Reading et al. 1991
Bufo marinusNo significant homing effect.Seebacher and Alford 1999
Bufo marinusReturn to their home site with 100% accuracy when displaced up to 70 m, or 165 m.Brattstrom 1962, Carpenter and Gillingham 1987
Bufo americanusReturned to within 5 m of their original site after a 235 m translocation.Dole 1972
Pseudacris triseriata triseriataThe majority of recaptures were within 100 m of the initial capture site.Kramer 1973
Pseudacris maculataThe maximum distance moved is only 250 m.Spencer 1964
Hyla regilla71% of males were found in the same portion of the same pond when recaptured the following year.Jameson 1957
Mixophyes iteratus89% of individuals were recaptured 5 m or less away from where they were marked.Lemckert and Brassil 2000
Rana sylvatica100% of adults and 82% of juvenile are faithful to their first breeding pond or natal pond respectively.Berven and Grudzien 1990
Rana sylvaticaThe mean distance between captures was only 11.25 m for 298 individuals.Bellis 1965
Rana pipiens98% of returned to their home pond after a one-kilometer displacement.Dole 1968
Rana muscosa89% percent of were recaptured at the same pond between breeding seasons.Pope and Matthews 2001
Rana muscosa50% of translocated individuals returned to their initial site within 20 days after a 630 m translocation.Matthews 2003
Rana lessonae and Rana ridibunda88% of individuals monitored between years did not move from their capture pond.Holenweg Peter 2001
Rana aurora draytonii75% did not move from their site over the course of a year and 90% of these philopatric individuals were never more than 60 m from the water.Bulger et al. 2003

Nevertheless, in our review we found that while the view of limited amphibian dispersal may be true for some species, it clearly does not hold for all. Among 166 journal articles concerning 90 species recording the maximum distance moved, while 44% of the amphibian species moved no farther than 400 m (Tables 3 and 4), 5% were capable of movements greater than 10 km (Table 5). This literature review sampled much more of total salamander diversity (37 species of 352 worldwide, 10.5%) than anuran diversity (53 species of 3848 worldwide, 1.5%) (Duellman and Trueb 1986), and there were evident differences between these groups. Nearly one half (44%) of the anuran species displayed maximum dispersal distances greater than one kilometre, and 7% of frogs were observed to have maximum dispersal distances greater than 10 km (Table 4). This is surprising considering that one kilometre has appeared independently in the literature as a magic number beyond which amphibian populations would be isolated from dispersal events (Berven and Grudzien 1990, Sjögren 1991, Waldick 1997, Vos and Chardon 1998, Newman and Squire 2001, Conroy and Brook 2003); and 15 km has been defined as the maximum migratory range (Sinsch 1990). Amphibian species truly do appear to be site-loyal on average, yet the frequency histogram of maximum distances moved is better fit by a power law than an exponential distribution (power (log-log) R2 = 0.67, exponential (log-normal) R2 = 0.25) (Figs. 1–3). In the power relationship, a proportion of the individuals are not described by that average and move long distances. This proportion is larger in the power relationship than in one described by an exponential function. If these long-distance-dispersers were frequent it would reduce the likelihood of support for the paradigm of metapopulation structure through isolation. Such a high rate of dispersal would effectively unite disjunct populations into a single unit – or patchy population ((Harrison 1991, Harrison and Taylor 1997) and not as Freckleton and Watkinson (2003)). If any metapopulation structure were to exist in this case, it would more likely be at a larger spatial scale than predicted by an exponentially distributed frequency distribution. Thus, it is incorrect to infer isolation from observed philopatry.

Table 5.  Summary table for the analysis of maximum reported distance moved for 164 studies of 90 species of amphibians.
 AMPHIBIANSFROGSSALAMANDERS
Number of species in dispersal studies905337
Number of dispersal studies16610264
% max dispersal≤1 km705694
% max dispersal≤400 m443164
% max dispersal≥10 km572
AVERAGE (m)2023.542922.51576.75
STANDARD DEVIATION (m)4895.615929.891664.92
VARIANCE (m2)2.40E+073.52E+072.77E+06
image

Figure 1. Frequency histogram of the maximum distance moved by amphibians from 166 journal articles (90 species). 200 m size bins. 30% of the reviewed studies had maximum movement distances >1 km. y=22.39x−0.7653, R2=0.7031.

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image

Figure 2. Frequency histogram of the maximum distance moved by salamander species from 62 journal articles (37 species). 200 m size bins. Six percent of the reviewed studies had maximum movement distances >1 km. y=6.23x−0.4823, R2=0.544

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image

Figure 3. Frequency histogram of the maximum distance moved by anurans from 102 journal articles (53 species). 200 m size bins. Forty-four percent of the reviewed studies had maximum movement distances >1 km. y=13.749x−0.6396, R2=0. 6797.

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Although 94% of the maximum dispersal distances for salamanders are less than 1 km (Table 3), the frequency distribution of distances was also an inverse power function (Fig. 2). Therefore, although most may not move very far, there is a strong likelihood that some individuals may complete long-distance movements. For example, Plethodon glutinosus is a remarkably site loyal animal across ages and sexes (Wells and Wells 1976). Using data provided in the paper for this species, we found that an inverse power law explained 74% of the variation in movement frequency for males (from Wells and Wells (1976)Fig. 1). In this case, although it is clear that most individuals are unlikely to move greater than 10 m, according to this relationship there is an appreciable chance (∼0.4%) that an individual could move one kilometre. Indeed, recent findings document that for some pond-breeding salamanders, rates of inter-pond migration are high enough to suggest that the fit of the metapopulation paradigm to this particular species was less than was expected (Trenham 1998).

Clearly, most amphibian species do not move very far, but surprisingly, greater than 7% of anurans we surveyed were capable of movements of greater than 10 km. This leptokurtic, inverse-power relationship neither supports nor rejects the model of the poorly dispersing amphibian. Concluding all amphibians are poor dispersers is as incorrect as stating that all mammals move long distances. Rather, the power relationship demonstrates that, as a group, amphibians exhibit a wide range of dispersal strategies. As has been demonstrated (Stumpel and Hanekamp 1986, Platx et al. 1990, Tunner 1992, Vos et al. 2000, Hayes et al. 2001, Smith 2003), some amphibian species are capable of movements that are surprising for presumably poorly dispersing animals. Our review suggests that anurans have an average maximum movement recorded (2.02 km) that is two times as large as the distance commonly reported as wide enough to result in population isolation. The diversity of maximum movement recorded is apparent in the wide variance (2.40 E +07). Indeed, the anuran average and variance are an order of magnitude larger than the same patterns in salamanders (Table 5). Fitting an inverse power law to the movement frequency patterns for anurans and salamanders results in the explanation of 70% and 55% of the variance. Specific expectations for an individual species should not be based on a pattern demonstrated using many species and independent studies. However, note that our analysis makes a general prediction regarding the spatial scale at which one should expect local population differentiation: that distance where the inverse power law predicts a number of migrants equal to one. Genetically, populations tend to be locally differentiated when Nem ≪1 (Kimura and Maruyama 1971). For salamanders, the inverse power law predicts that at least one individual is likely to move distances less than 8–9 km and for anurans at least one individual is likely to move 11-13 km. When pond networks are separated by these approximate distances – where dispersal is possible, but not common – is more likely to be a scale where the simple, stochastic metapopulation approach is applicable for amphibians. Such a rule will obviously not hold for all species all of the time (the environment between habitat patches, the species, the condition of individuals will all affect dispersal abilities and will differ), but our generalization is a hypothesis informed by the literature that invites further testing.

Others have noted that the perception of limited amphibian dispersal may reflect the scale at which amphibian-researchers operate, rather than the scale at which amphibians disperse (Turner 1960, Dole 1971, Staub et al. 1995, Marsh et al. 1999, Pope et al. 2000). Among those studies where there was enough data reported to estimate the longest axis of the study area, that distance was therefore the longest possible distance that could have been recorded. A regression of maximum distance dispersed on the size of the studies long axis resulted in a significant positive relationship where 72.65% of the observed variance in maximum dispersal distance is explained by having a larger study site (Fig. 4). This relationship suggests that our understanding of the maximum distances amphibians can move is being underestimated by the site size where we study such movement. Our review makes a simple prediction that monitoring larger areas in the future will result in the discovery of longer distance movements for both anurans and salamanders.

image

Figure 4. Relating the maximum movement recorded with the maximum size of the study area.

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Case study

  1. Top of page
  2. Abstract
  3. Amphibians and Hanski's four metapopulation conditions
  4. Amphibian site loyalty and movement
  5. Case study
  6. Conclusions
  7. Acknowledgements
  8. References
  9. Appendix

Some amphibians do function as metapopulations (Gill 1978, Sjögren 1991, Sjögren Gulve and Ray 1996, Vos et al. 2000). However, examining the amphibian-as-metapopulation literature indicates that the generalization that all amphibians will operate as metapopulations (Alford and Richards 1999) is not supported. The majority of the metapopulation conditions are either not tested or are assumed to hold in the amphibian as metapopulation literature. Instead, it is often taken as a foregone conclusion that amphibians operate as metapopulations. Bulger et al. (2003), for example, invoke metapopulation structure without having tested any of the related (Hanski and Kuussaari 1995, Hanski et al. 1995, Hanski 1999) hypotheses. For species we have examined ourselves (Bufo americanus, Bufo fowleri, Pseudacris crucifer, Rana sylvatica, Rana pipiens, Rana clamitans and Rana catesbeiana), both locally (on a scale of ≤10 km) and regionally (for B. fowleri≤300 km), we found no evidence of metapopulation structure at either scale, and concluded that while there was not enough movement between populations separated by many tens of kilometres to support a metapopulation effect, there was likely too much dispersal occurring between the habitat patches separated by 10 km or less (Smith 2003). Most of these species are common to the area we investigated, but B. fowleri is currently listed as Threatened by the Committee on the Status of Endangered Species (COSEWIC) in Canada largely due to its isolated and fragmented habitat. Indeed, a metapopulation based conservation approach was intuitively appealing for this species, as, prior to our analysis, it qualitatively appeared to meet all of Hanski's four conditions.

To reconcile the acceptance of the metapopulation approach in amphibian conservation and ecology with the lack of stringent testing of hypotheses we compared those characteristics common to amphibians as metapopulations (Marsh and Trenham 2001), 1) population dynamics determined by pond-based-processes, 2) common local extinction and colonisation, 3) local extinction occurring in suitable habitat, 4) limited dispersal causing isolation) to the studies we have reviewed and the species for which we have data.

The majority (33/53) of the studies we examined had either implicitly assumed, or had tested, that the “ponds as patches” view was an accurate depiction of an amphibian population. Recently, Skelly and Meir (1997), Pope et al. (2000) and Marsh and Trenham (2001) have criticized the assumed primacy of the pond. For instance, with Rana pipiens, any apparent metapopulation structure was removed when the non-pond variable of “summer habitat” was removed from the analysis (Pope et al. 2000). In our own work with B. fowleri, we know that the number of non-reproductive one year olds at year (t) explains nearly 80% of the variation in captured adults in year (t+1) (Green and Smith unpubl.). Although strong, this relationship was derived using only 5 yr worth of data and excludes one year where it was suspected that migration boosted the observed number of reproductive animals in year (t+1). Thus while population processes occurring in the pond are clearly important, there is strong evidence that events occurring outside the pond (migration, over-wintering success) also contribute the observed population dynamics. Therefore, instead of asking is the pond a patch (∼Hanski Condition 1), we should ask is the patch only the pond? Stated another way; perhaps the easily spatially delineated feature “pond” is not the disjunct habitat one should model. The application to amphibian conservation is clear, for if one adopted a metapopulation based conservation approach on the hypothesis of ponds as metapopulation patches – and summarily protected those patches – without testing whether the habitat critical to the species survival was actually described by pond boundaries it is possible that the truly important habitat would not be protected.

Forty-five out of 53 articles in the literature (85%) did not examine the frequency of local extinction and colonization. A limited number of between-year occupancy transitions severely restrict the researcher's ability to determine whether extinctions and colonizations are actually common features of the network of breeding assemblages (Thomas et al. 2002). For instance, within the local habitat patch assemblage we monitor in Ontario, there have been 38 observed colonisation and 31 extinction events for B. fowleri measured over 15 y at Long Point, resulting in average colonisation and extinction rates of 0.2798 and 0.2481 respectively. Compared to values for other amphibian species (Table 2 of Marsh and Trenham (2001)), the values for B. fowleri are amongst the highest and yet there is no evident metapopulation effect involved in B. fowleri population dynamics (Smith 2003). Due to relatively high rates of local extinction and colonisation in B. fowleri, a conservation strategy might be based on the faulty notion that these toads occupy local and regional metapopulations when they are actually more intimately connected by regular dispersal (i.e. a patchy population).

One finding recorded regularly in the literature was that local amphibian extinctions were deterministic, not stochastic, as habitats underwent succession (Sjögren 1991, Skelly et al. 1999, Marsh and Trenham 2001, Bradford et al. 2003). Indeed, at Long Point the environment is constantly undergoing succession, and ponds are likely to be exposed to deterministic degradation (as B. fowleri breeding habitat) as they go through succession. We suspected that at least several of the observed extinctions at Long Point were due to succession reducing habitat quality to such an extent as to cause extinction. Perhaps the effects of this deterministic change are predominant over any stochastic changes we have measured (Skelly et al. 1999), but a quantitative analysis of this question is beyond the scope of this investigation. Clearly though it remains important to do so (Ellner and Fussmann 2003), as differentiating between the importance of stochastic and deterministic changes will inform conservation decisions regarding whether management should focus on landscape factors or local habitat conditions.

The supposition of limited dispersal causing isolation was the most frequently implicated reason for evident, or assumed, metapopulation structure in amphibians. In our own work with B. fowleri, we concluded that dispersal was likely the primary factor implicated in the lack of metapopulation structure we demonstrated – both too little and too frequent (Smith 2003). At a local scale of ≤10 km, individuals dispersed over a distance and at a rate that made even isolated populations connected to the whole. Regionally (≤300 km), populations were too isolated for even occasional migrants to recolonise habitat following local extinction. We suspect that the generalization of limited amphibian dispersal causing population isolation, and therefore metapopulation structure, may not be warranted as frequently as the literature implies (Alford and Richards 1999). This is especially true for pond-breeding anurans species with a high turnover of local populations and dependent upon dispersal for its persistence, for without the effect of rescue from neighboring populations, they will suffer greater cumulative local extinctions (Green 2003). We find that movement distances for anurans are an order of magnitude greater (∼10 km) than has previously been thought.

Conclusions

  1. Top of page
  2. Abstract
  3. Amphibians and Hanski's four metapopulation conditions
  4. Amphibian site loyalty and movement
  5. Case study
  6. Conclusions
  7. Acknowledgements
  8. References
  9. Appendix

Our review demonstrates that the applicability of the metapopulation paradigm to amphibian species is largely dependant on the hypothesis of limited dispersal. As there are a wide range of dispersal abilities within amphibian species, we should be cautious with the indiscriminant application of the metapopulation approach to amphibians – especially where conservation decisions are to be based on the assumptions of isolation and metapopulation structure though limited dispersal. Although amphibians are predominantly site-loyal and of low vagility, they can move distances much greater than previously anticipated. Their dispersal capabilities suggest that occasional migrants may connect populations separated by tens of kilometers. Although it is clear that type and quality of the landscape occurring between habitat patches will affect the number of successful immigrants (Ray et al. 2002); we make the general suggestion that for salamanders and anurans, population differentiation is most likely to occur at scales upward of 10 km. If somewhat regular movement of individuals can connect populations separated by distances smaller than this, then the effective number of populations is reduced. If reduced to one, then simple patch occupancy models are ineffective tools for research or conservation as they ignore local dynamics (Hanski 1998). All amphibians are not metapopulations and not all amphibians are dispersal poor. The paradigm of pond-breeding amphibian populations as metapopulations has been adopted before there have been sufficient data available to evaluate it (Hanski and Simberloff 1997). If the meaning of the term “metapopulation” has lost clarity (Smedbol et al. 2002), researchers would do well to test the elementary predictions of a simple metapopulation prior to announcing that their particular study organism/population constitutes one. If a researcher were encouraged to use the term metapopulation without even simple tests of whether necessary conditions were met, then the metapopulation would no longer be a falsifiable hypothesis. We feel that the metapopulation concept has much greater utility, and scientific integrity, when it is phrased as a hypothesis.

It is not our intent to summarily reject the positive influence the development of the metapopulation concept has had on ecology and conservation. Undoubtedly there are amphibian species for which the metapopulation assumption of pond isolation due to limited dispersal is valid. However, the generality of this assumption is not supported by data. The metapopulation approach – especially regarding habitat patch isolation due to limited dispersal – must be more stringently tested and more clearly reported.

Acknowledgements

  1. Top of page
  2. Abstract
  3. Amphibians and Hanski's four metapopulation conditions
  4. Amphibian site loyalty and movement
  5. Case study
  6. Conclusions
  7. Acknowledgements
  8. References
  9. Appendix

We gratefully acknowledge the support of the Natural Sciences and Engineering Council (NSERC) and the World Wildlife Fund Canada Endangered Species Recovery Fund for grants to DMG and the Mountain Equipment Co-op Environment Fund, McGill Univ., NSERC and Fonds Nature et Technologies for grants and scholarships to MAS. Comments from T. Hels helped improve this manuscript. M.A.S. gives special thanks to Elaine Bazinet Smith, Clark Smith and Alison Judd for support and encouragement.

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  3. Amphibians and Hanski's four metapopulation conditions
  4. Amphibian site loyalty and movement
  5. Case study
  6. Conclusions
  7. Acknowledgements
  8. References
  9. Appendix
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Appendix

  1. Top of page
  2. Abstract
  3. Amphibians and Hanski's four metapopulation conditions
  4. Amphibian site loyalty and movement
  5. Case study
  6. Conclusions
  7. Acknowledgements
  8. References
  9. Appendix

Appendix A: Appendix 1. Testing of metapopulation conditions. 0=not tested, 1=tested, and 2=assumed.

Table Text Table 6. . 
Condition 1: habitat patches support local breeding populationsCondition 2: no single population is large enough to ensure long-term survivalCondition 3: patches are not too isolated to prevent recolonisation (i.e. limited dispersal allows isolation with potential recolonisation)Condition 4: local dynamics are sufficiently asynchronous to make simultaneous extinction of all local populations unlikelySource
2222Alford and Richards 1999
1110Berven and Grudzien 1990
2120Blaustein et al. 1994
2110Bradford et al. 2003
2010Call 1997
2020Carlson and Edenhamn 2000
2220Corser 2001
0010Driscoll 1997
1010Gill 1978
2020Goldber 2002
2020Halley et al. 1996
1120Hartwell 1990
2020Hecnar and M'Closkey 1996
0010Hels 2002
2020Hranitz and Diehl 2000
0010Johnson and Semlitsch 2003
2010Knapp et al. 2003
2222Laan and Verboom 1990
0210Marsh et al. 1999
0020Marsh and Trenham 2001
2010Monsen 2002
0010Muths et al. 2003
2212Newman and Squire 2001
0010Osborne and Norman 1991
1020Perret et al. 2003
0020Pope et al. 2000
0010Reading et al. 1991
2212Reh and Seitz 1990
2010Ritland et al. 2000
0020Routman 1993
2012Rowe et al. 2000
0010Scribner et al. 1994
0010Scribner et al. 2001
0020Semlitsch and Bodie 1998
0020Semlitsch et al. 1996
2010Seppa and Laurila 1999
0010Shaffer et al. 2000
0010Sinsch 1992
1110Sjögren 1991
1110Sjögren Gulve 1994
0011Skelly and Meir 1997
2010Skelly et al. 1999
0010Tallmon et al. 2000
1111Ter Braak and Etienne 2003
1110Trenham 1998
2010Vos et al. 2001
2010Vos and Chardon 1998
1111Vos et al. 2000
0020Woodford and Meyer 2003
2020Conroy and Brook 2003
0020Ebisuno and Gentilli 2002
1020Joly et al. 2001