Present address: Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.
Stress-induced apoptosis in larval embryos of Japanese flounder
Article first published online: 28 NOV 2003
Volume 69, Issue 6, pages 1218–1223, December 2003
How to Cite
YABU, T., ISHIBASHI, Y. and YAMASHITA, M. (2003), Stress-induced apoptosis in larval embryos of Japanese flounder. Fisheries Science, 69: 1218–1223. doi: 10.1111/j.0919-9268.2003.00748.x
- Issue published online: 28 NOV 2003
- Article first published online: 28 NOV 2003
- Received 26 December 2002. Accepted 22 May 2003.
- heat shock;
- Paralichthys olivaceus;
ABSTRACT: Stress-induced apoptosis was characterized in larval embryos of Japanese flounder using a caspase-3-like activity assay and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining of whole mounts. Severe stress conditions, such as heat shock by temperature shift from 16°C to 28°C or 29°C for 1 h, and ultraviolet (UV) irradiation at 20–100 mJ/cm2, induced extensive apoptosis in embryos. Following the induction of stress, many TUNEL-positive apoptotic cells were found in the yolk sac, heart, and larval fin. Caspase-3-like activity against a fluorogenic substrate, acetyl-Asp-Glu-Val-Asp-α-4-methylcoumaryl-7-amide, increased in a dose-dependent manner during stress-induced apoptosis. In addition, C2-ceramide treatment (5–10 µM) induced a similar apoptosis pattern to stress treatment. These findings indicate that a pro-apoptotic pathway, which is caspase-3 mediated and acts through ceramide signaling, plays a major role in induction of apoptosis under stress conditions.