Both authors contributed equally to this work.
The vanishing clone: karyotypic evidence for extensive intraclonal genetic variation in the peach potato aphid, Myzus persicae (Hemiptera: Aphididae)
Article first published online: 16 DEC 2011
© 2011 The Linnean Society of London
Biological Journal of the Linnean Society
Volume 105, Issue 2, pages 350–358, February 2012
How to Cite
MONTI, V., MANDRIOLI, M., RIVI, M. and MANICARDI, G. C. (2012), The vanishing clone: karyotypic evidence for extensive intraclonal genetic variation in the peach potato aphid, Myzus persicae (Hemiptera: Aphididae). Biological Journal of the Linnean Society, 105: 350–358. doi: 10.1111/j.1095-8312.2011.01812.x
- Issue published online: 11 JAN 2012
- Article first published online: 16 DEC 2011
- Received 20 July 2011; revised 8 September 2011; accepted for publication 8 September 2011
- fragile sites;
- holocentric chromosomes;
- repetitive DNAs;
Analysis of holocentric mitotic metaphase chromosomes of the peach-potato aphid Myzus persicae (Sulzer) clone 33H revealed different chromosome numbers, ranging from 12 to 17 within each embryo, in contrast to the standard karyotype of this species (2n = 12). Chromosome length measurements revealed that the observed chromosomal mosaicism is the result of recurrent fragmentations of chromosomes X, 1 and 3 because of fragile sites or hot spots of recombination. Fluorescent in situ hybridization experiments showed that X chromosomes were frequently involved in recurrent fragmentations, in particular their telomeric end opposite to the nucleolar organizer region. Experiments to induce males showed that M. persicae clone 33H is obligately parthenogenetic. The reproduction by apomictic parthenogenesis, together with a high telomerase expression that stabilized the chromosomes involved in the fragmentations observed in the M. persicae clone 33H, appears to favour the stabilization of the observed chromosome instability. © 2011 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, 105, 350–358.