Figure S1. Morphometry of the first lower molar of Microtus with the 27 measurements.


Figure S2. Graphical representation of arvalis demographic history. Observed mismatch distributions (grey line) for each lineage are compared with the expected distributions (black line) under a population growth–decline model.


Figure S3. Canonical discriminant analyses showing the distribution of the centroids on the two first axes for the different geographical populations.


Figure S4. Bayesian tree reconstructed from a 743-bp long fragment of arvalis and obscurus cytochrome b gene from our sequence dataset and F1 and F2 sequences from Bulatova et al. (2010b). Sequences reconstructed from Bulatova et al. (2010b) are marked with an asterisk (*) and those in red are karyotyped obscurus individuals with arvalis mitochondrial haplotypes. Numbers at nodes refer to Bayesian inference posterior probabilities (≥ 0.70). Lineages are indicated on the right: in black for arvalis lineages and in grey for obscurus ones.


Table S1. Labels, geographical distribution, and references or tissue providers of obscurus and arvalis samples; accession numbers for original and GenBank data, as well as cytochrome b gene haplotypes, are also listed.

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