Get access

Cytogenetic and molecular characterization of morphologically variable Sisyrinchium micranthum (Iridaceae) in southern Brazil

Authors

  • LUANA O. TACUATIÁ,

    Corresponding author
    1. Instituto de Biociências, Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves 9500, Prédio 43323M, C.P. 15053, Porto Alegre, RS, 91501-970, Brazil
    Search for more papers by this author
  • TATIANA T. SOUZA-CHIES,

    1. Instituto de Biociências, Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves 9500, Prédio 43323M, C.P. 15053, Porto Alegre, RS, 91501-970, Brazil
    2. Instituto de Biociências, Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves 9500, Prédio 43433, Porto Alegre, RS, 91501-970, Brazil
    Search for more papers by this author
  • ALICE M. FLORES,

    1. Instituto de Biociências, Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves 9500, Prédio 43433, Porto Alegre, RS, 91501-970, Brazil
    2. Instituto de Biociências, Departamento de Genética, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves 9500, Prédio 43323M, C.P. 15053, Porto Alegre, RS, 91501-970, Brazil
    Search for more papers by this author
  • LILIAN EGGERS,

    1. Instituto de Biociências, Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves 9500, Prédio 43433, Porto Alegre, RS, 91501-970, Brazil
    Search for more papers by this author
  • SONJA SILJAK-YAKOVLEV,

    1. Université Paris-Sud, Ecologie, Systématique, Evolution, UMR 8079 CNRS-UPS-AgroParisTech, Bâtiment 360, 91450 Orsay, France
    Search for more papers by this author
  • ELIANE KALTCHUK-SANTOS

    1. Instituto de Biociências, Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves 9500, Prédio 43323M, C.P. 15053, Porto Alegre, RS, 91501-970, Brazil
    2. Instituto de Biociências, Departamento de Genética, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves 9500, Prédio 43323M, C.P. 15053, Porto Alegre, RS, 91501-970, Brazil
    Search for more papers by this author

E-mail: tacuatia@yahoo.com.br

Abstract

Sisyrinchium micranthum is native to southern Brazil and shows a wide range of morphological variation in this region. This study was performed to compare individuals of this species in terms of traits such as morphology, chromosome number, meiotic behaviour, pollen viability and genetic diversity using inter simple sequence repeat markers. First, general morphological aspects were evaluated and plants were classified into three morphological categories (CI, CII and CIII) to analyse the cytogenetic and genetic data. Three chromosome numbers corresponding to three ploidies were found: 2n = 2x = 16, 2n = 4x = 32 and 2n = 6x = 48. All morphological types showed regular meiotic behaviour and high meiotic index values and pollen viability. CII is the most frequent in southern Brazil. The most common haploid chromosome number, n = 8, has not been reported previously in this species. The percentage of polymorphic loci ranged from 6.45 to 61.29% for each accession. Molecular and cytogenetic analyses indicate that some S. micranthum accessions may have more than one ploidy. This study represents the first effort to characterize this taxonomically complex species based on cytogenetic and genetic aspects. © 2012 The Linnean Society of London, Botanical Journal of the Linnean Society, 2012, 169, 350–364.

Ancillary