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A simplified protocol for routine total DNA isolation from salmonid fishes

Authors

  • J. B. Taggart,

    1. Division of Environmental and Evolutionary Biology, School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland, U.K.
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  • R. A. Hynes,

    1. Division of Environmental and Evolutionary Biology, School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland, U.K.
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  • P. A. Prodöuhl,

    1. Division of Environmental and Evolutionary Biology, School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland, U.K.
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  • A. Ferguson

    1. Division of Environmental and Evolutionary Biology, School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland, U.K.
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Abstract

An efficient total DNA isolation protocol, suitable for routine population genetic screening purposes is described. This phenol based extraction can utilize fresh, frozen or ethanol preserved tissues.

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