Rapid HPLC analysis of retinoids and dehydroretinoids stored in fish liver: comparison of two lake sturgeon populations

Authors

  • C. Doyon,

    1. Centre de recherche TOXEN and Département des sciences biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre-Ville, Montréal, QC, Canada H3C 3P8
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  • S. Boileau,

    1. Centre de recherche TOXEN and Département des sciences biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre-Ville, Montréal, QC, Canada H3C 3P8
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  • R. Fortin,

    1. Centre de recherche TOXEN and Département des sciences biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre-Ville, Montréal, QC, Canada H3C 3P8
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  • P. A. Spear

    Corresponding author
    1. Centre de recherche TOXEN and Département des sciences biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre-Ville, Montréal, QC, Canada H3C 3P8
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Author to whom correspondence should be addressed. Tel.: 514 987 3000 ext. 4346; fax: 514 987 4647; email: toxen@nobel.si.uqam.ca

Abstract

All major retinoids (vitamin A) stored in the liver of lake sturgeon from two Québec lakes were analysed by high-performance liquid chromatography (HPLC). Four HPLC peaks represented approximately 90% of all liver retinoids. Their relative proportion was remarkably consistent in the two sturgeon populations with dehydroretinyl palmitate/oleate accounting for the largest proportion (50–60%). Most retinoids demonstrated significant positive correlations with age, but only in the Lake Berthelot sturgeon. The concentrations of all major retinoids were significantly (P<0·0001) lower, as much as 40 times lower, in the Lake Saint-Louis population. Possible explanations for the extremely low liver stores in the Lake Saint-Louis sturgeon are discussed.

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