Comparison of genetic diversity at microsatellite loci in near-extinct and non-endangered species of Mexican goodeine fishes and prediction of cross-amplification within the family

Authors

  • R. M. Hamill,

    Corresponding author
    1. * Harold Mitchell Building, School of Biology, University of St Andrews, Fife, KY16 9TH, Scotland, U.K. and Departamento de Ecología Evolutiva, Instituto de Ecología, UNAM, A. P. 70–275, C. P. 04510, Coyoacán, D. F., México
      †Tel.: +44 (0)1334 463495; fax: +44 (0)1334 463366; email: rh30@st-and.ac.uk
    Search for more papers by this author
  • S. A. Webb,

    1. * Harold Mitchell Building, School of Biology, University of St Andrews, Fife, KY16 9TH, Scotland, U.K. and Departamento de Ecología Evolutiva, Instituto de Ecología, UNAM, A. P. 70–275, C. P. 04510, Coyoacán, D. F., México
    Search for more papers by this author
  • C. Macías Garcia,

    1. * Harold Mitchell Building, School of Biology, University of St Andrews, Fife, KY16 9TH, Scotland, U.K. and Departamento de Ecología Evolutiva, Instituto de Ecología, UNAM, A. P. 70–275, C. P. 04510, Coyoacán, D. F., México
    Search for more papers by this author
  • J. A. Graves,

    1. * Harold Mitchell Building, School of Biology, University of St Andrews, Fife, KY16 9TH, Scotland, U.K. and Departamento de Ecología Evolutiva, Instituto de Ecología, UNAM, A. P. 70–275, C. P. 04510, Coyoacán, D. F., México
    Search for more papers by this author
  • A. E. Magurran,

    1. * Harold Mitchell Building, School of Biology, University of St Andrews, Fife, KY16 9TH, Scotland, U.K. and Departamento de Ecología Evolutiva, Instituto de Ecología, UNAM, A. P. 70–275, C. P. 04510, Coyoacán, D. F., México
    Search for more papers by this author
  • M. G. Ritchie

    1. * Harold Mitchell Building, School of Biology, University of St Andrews, Fife, KY16 9TH, Scotland, U.K. and Departamento de Ecología Evolutiva, Instituto de Ecología, UNAM, A. P. 70–275, C. P. 04510, Coyoacán, D. F., México
    Search for more papers by this author

†Tel.: +44 (0)1334 463495; fax: +44 (0)1334 463366; email: rh30@st-and.ac.uk

Abstract

The cross-utility of 12 microsatellite loci (including nine newly developed loci) amongst the viviparous subfamily, the Mexican Goodeinae, was assessed, examining both the probability of amplification and the potential incidence of null alleles from tests of FIS. Genetic diversity was relatively high in comparison to other freshwater species. Amplification success was not correlated with genetic distance between microsatellite source and target species, but taxa that were more distantly related were less likely to be cross-polymorphic for microsatellite loci. On average, species that were cross-polymorphic were separated by a genetic distance of 15% at the cytochrome oxidase I locus, while those that were monomorphic were separated by 19%. There was no evidence that null alleles become more frequent at greater source-target genetic distance.

Ancillary