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Genetic structure of the diploid–polyploid fish Squalius alburnoides in southern Iberian basins Tejo and Guadiana, based on microsatellites

Authors

  • M. E. Crespo-López,

    1. * Universidade de Lisboa, Faculdade de Ciências, Centro de Biologia Ambiental/Departamento de Biologia Animal, da, Bloco C2-3° piso, Campo Grande, Portugal and § School of Life Sciences, P. O. Box 874501, Arizona State University, Tempe, Az 85287-4501, U.S.A.
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    • Present address: Laboratório de Farmacologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Campus do Guamá, Av. Augusto Côrrea, 01, 66075-110 Belém (PA), Brazil.

  • I. Pala,

    1. * Universidade de Lisboa, Faculdade de Ciências, Centro de Biologia Ambiental/Departamento de Biologia Animal, da, Bloco C2-3° piso, Campo Grande, Portugal and § School of Life Sciences, P. O. Box 874501, Arizona State University, Tempe, Az 85287-4501, U.S.A.
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  • T. L. Duarte,

    1. * Universidade de Lisboa, Faculdade de Ciências, Centro de Biologia Ambiental/Departamento de Biologia Animal, da, Bloco C2-3° piso, Campo Grande, Portugal and § School of Life Sciences, P. O. Box 874501, Arizona State University, Tempe, Az 85287-4501, U.S.A.
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    • Present address: Radiation and Oxidative Stress Section, Department of Cancer Studies and Molecular Medicine, University of Leicester, Robert Kilpratick Clinical Science Building, Leicester Royal Infirmary, Leicester LE2 7LX, U.K.

  • T. E. Dowling,

    1. * Universidade de Lisboa, Faculdade de Ciências, Centro de Biologia Ambiental/Departamento de Biologia Animal, da, Bloco C2-3° piso, Campo Grande, Portugal and § School of Life Sciences, P. O. Box 874501, Arizona State University, Tempe, Az 85287-4501, U.S.A.
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  • M. M. Coelho

    Corresponding author
    1. * Universidade de Lisboa, Faculdade de Ciências, Centro de Biologia Ambiental/Departamento de Biologia Animal, da, Bloco C2-3° piso, Campo Grande, Portugal and § School of Life Sciences, P. O. Box 874501, Arizona State University, Tempe, Az 85287-4501, U.S.A.
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‖Centro de Biologia Ambiental/Departamento de Zoologia e Antropologia, Faculdade de Ciências da Universidade de Lisboa, Bloco C2-3° piso, Campo Grande, 1749-016 Lisboa, Portugal. Tel.: +351 21 7500000; fax: +351 21 7500028; email: mmcoelho@fc.ul.pt

Abstract

Several studies have demonstrated the hybrid origin of Squalius alburnoides, a complex of diploid, triploid and tetraploid fish, with Squalius pyrenaicus as the maternal ancestor and an Anaecypris hispanica-like species as the paternal ancestor. The aim of this study was to assess patterns of genetic diversity and similarity between sympatric forms of the different ploidy levels of S. alburnoides and related parental species in the two largest basins that are part of its southern Iberian distribution, the Tejo and Guadiana, using microsatellites. High levels of genetic diversity were found in populations of S. alburnoides from both basins, reflecting the hybrid origin, the high diversity of forms in these river basins and the different modes of reproduction of the complex. Microsatellites indicated low values of genetic differentiation between the non-hybrid all-male form of S. alburnoides and A. hispanica, supporting the view that these forms share a common ancestor. In some instances, diploid and triploid hybrids from the same river basin were more closely related to each other than to their counterparts in the other basins, suggesting an ancient origin for the complex in these Iberian southern basins.

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